Takahashi H
Department of Urology, Hiroshima University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1998 Oct;89(10):836-45. doi: 10.5980/jpnjurol1989.89.836.
We tried to clarify the role of fibroblast growth factors (FGFs) and those receptors (FGF-Rs) in cell proliferation of human prostate cancer.
The mRNA expression of FGF1, FGF2, FGF7, FGF-R1, FGF-R2 (IIIb), and FGF-R2 (IIIc) was investigated by RT-PCR in androgen sensitive cells (LNCaP), androgen-independent cells (PC3) and primary cultured stromal (PS) and epithelial cells (PE) from benign prostatic hyperplasia (BPH). Expression of the mRNA of FGF-R1, FGF-R2 (IIIb) and FGF-R2 (IIIc) in human prostate cancer tissue was similarly analyzed. Furthermore, the level of FGF-R1 expression in human prostate cancer was measured by semi-quantitative RT-PCR.
FGF-R1 mRNA was detected in LNCaP, PC3 and the primary cultured stromal cells of BPH. FGF-R2 (IIIb) was seen in LNCaP cells and the primary cultured epithelial cells of BPH, while FGF-R2 (IIIc) was only observed in PC3. FGF1 mRNA was expressed in LNCaP and PC3, while FGF2 mRNA was in PC3 alone. The expression of FGF7 mRNA was detected only in the primary cultured stromal cells. Of 17 patients with human prostate cancer, FGF-R2 (IIIb) was detected in 2 and FGF-R2 (IIIc) in 15. Histological type of two cases having FGF-R2 (IIIb) were well differentiated adenocarcinoma. The mRNA levels of FGF-R1 in poorly and moderately differentiated types were significantly higher than those in well differentiated ones (p < 0.05).
These findings suggest that several changes of expression in FGFs and FGF-Rs may correlate with malignant progression of human prostate cancer.
我们试图阐明成纤维细胞生长因子（FGFs）及其受体（FGF-Rs）在人前列腺癌细胞增殖中的作用。
通过逆转录聚合酶链反应（RT-PCR）研究雄激素敏感细胞（LNCaP）、雄激素非依赖细胞（PC3）以及来自良性前列腺增生（BPH）的原代培养基质细胞（PS）和上皮细胞（PE）中FGF1、FGF2、FGF7、FGF-R1、FGF-R2（IIIb）和FGF-R2（IIIc）的mRNA表达。同样分析人前列腺癌组织中FGF-R1、FGF-R2（IIIb）和FGF-R2（IIIc）的mRNA表达。此外，通过半定量RT-PCR检测人前列腺癌中FGF-R1的表达水平。
在LNCaP、PC3和BPH的原代培养基质细胞中检测到FGF-R1 mRNA。在LNCaP细胞和BPH的原代培养上皮细胞中可见FGF-R2（IIIb），而FGF-R2（IIIc）仅在PC3中观察到。FGF1 mRNA在LNCaP和PC3中表达，而FGF2 mRNA仅在PC3中表达。FGF7 mRNA的表达仅在原代培养的基质细胞中检测到。在17例人前列腺癌患者中，2例检测到FGF-R2（IIIb），15例检测到FGF-R2（IIIc）。具有FGF-R2（IIIb）的2例的组织学类型为高分化腺癌。低分化和中分化类型中FGF-R1的mRNA水平显著高于高分化类型（p < 0.05）。
这些发现表明FGFs和FGF-Rs表达的若干变化可能与人前列腺癌的恶性进展相关。
