Zattara-Cannoni H, Dufour H, Lepidi H, Chatel C, Grisoli F, Vagner-Capodano A M
Cytogenetic Oncology Laboratory, C.H.U. TIMONE, Marseille, France.
Cancer Genet Cytogenet. 1998 Dec;107(2):98-101. doi: 10.1016/s0165-4608(98)00095-8.
Cytogenetic analysis provides important information for diagnosis and prognosis in some tumors. But karyotype analysis can be difficult in some cases, because metaphase chromosomes are contracted. New techniques, such as fluorescence in situ hybridization and, more recently, spectral karyotyping, or SKY, based on the hybridization of 24 fluorescently labeled chromosome painting probes, allow the detection and identification of complex chromosomal rearrangements. We report here a case of primary central nervous system lymphoma in which chromosomal rearrangements and marker chromosomes not identified by a routine cytogenetic technique were clarified by SKY. This shows the value of the SKY technique in the cytogenetic diagnosis of tumors.
细胞遗传学分析为某些肿瘤的诊断和预后提供重要信息。但在某些情况下，核型分析可能会很困难，因为中期染色体是收缩的。新技术，如荧光原位杂交，以及最近基于24种荧光标记染色体涂染探针杂交的光谱核型分析（SKY），能够检测和识别复杂的染色体重排。我们在此报告一例原发性中枢神经系统淋巴瘤病例，其中常规细胞遗传学技术未识别出的染色体重排和标记染色体通过SKY得以明确。这显示了SKY技术在肿瘤细胞遗传学诊断中的价值。
