Anthony P, Davey M R, Power J B, Lowe K C
Department of Life Science, University of Nottingham, U.K.
Artif Cells Blood Substit Immobil Biotechnol. 1998 Nov;26(5-6):449-54. doi: 10.3109/10731199809117465.
Cell suspension-derived protoplasts of Petunia hybrida cv. Comanche were cultured for up to 10 d in (1) aqueous medium, (2) aqueous medium overlaying an oxygenated (10 mbar, 15 min) perfluorochemical liquid (perfluorodecalin; PFC), (3) aqueous medium containing 1:50 (v:v) of the commercial haemoglobin (Hb) solution (Erythrogen), (4) aqueous medium supplemented with 1:50 (v:v) of Erythrogen overlaying oxygenated PFC. Treatments 2, 3, and 4 enhanced mean mitotic division by 111%, 80% and 139% respectively, compared to controls. Both fresh-Hb and stored-Hb significantly (P < 0.05) enhanced mitotic division, although this was 45% greater with fresh-Hb compared to stored-Hb. Both oxygenated PFC and Erythrogen provide approaches for enhancing cellular oxygen supply to eukaryotic cells. Commercially, the recoverability and recycleability of PFCs make these compounds a more attractive option in comparison to Erythrogen, despite a high initial investment cost.
对矮牵牛品种科曼奇（Petunia hybrida cv. Comanche）的细胞悬浮液来源的原生质体进行了长达10天的培养，培养条件如下：（1）水相培养基；（2）覆盖有经氧合处理（10毫巴，15分钟）的全氟化合物液体（全氟萘烷；PFC）的水相培养基；（3）含有1:50（体积比）商业血红蛋白（Hb）溶液（促红素）的水相培养基；（4）覆盖有经氧合处理的PFC且添加了1:50（体积比）促红素的水相培养基。与对照组相比，处理2、3和4分别使平均有丝分裂增加了111%、80%和139%。新鲜Hb和储存Hb均显著（P<0.05）提高了有丝分裂，不过新鲜Hb比储存Hb的提高幅度大45%。经氧合处理的PFC和促红素都为增强向真核细胞的细胞氧供应提供了方法。在商业上，尽管PFC的初始投资成本很高，但PFC的可回收性和可循环利用性使其比促红素更具吸引力。
