小鼠核孤儿受体GCNF在初级精母细胞的XY小体中表达。

The murine nuclear orphan receptor GCNF is expressed in the XY body of primary spermatocytes.

作者信息

Bauer U M, Schneider-Hirsch S, Reinhardt S, Benavente R, Maelicke A

机构信息

Laboratory of Molecular Neurobiology, Institute of Physiological Chemistry and Pathobiochemistry, Johannes Gutenberg-University Medical School, Mainz, Germany.

出版信息

FEBS Lett. 1998 Nov 20;439(3):208-14. doi: 10.1016/s0014-5793(98)01327-1.

DOI:10.1016/s0014-5793(98)01327-1

PMID:9845324

Abstract

We have studied the expression of the nuclear orphan receptor GCNF (germ cell nuclear factor) on the mRNA and protein level in pubertal and adult mouse testes. We show by Northern and Western blot analyses and by in situ hybridization that GCNF is expressed in spermatocytes and round spermatids of adult mouse testis suggesting that GCNF may be a transcriptional regulator of spermatogenesis. Since the GCNF protein is accumulated in the XY body of late pachytene spermatocytes, it may be involved in transcriptional inactivation of sex chromosomes.

摘要

我们研究了核孤儿受体GCNF（生殖细胞核因子）在青春期和成年小鼠睾丸中mRNA和蛋白质水平的表达。通过Northern和Western印迹分析以及原位杂交，我们发现GCNF在成年小鼠睾丸的精母细胞和圆形精子细胞中表达，这表明GCNF可能是精子发生的转录调节因子。由于GCNF蛋白在粗线期晚期精母细胞的XY小体中积累，它可能参与性染色体的转录失活。