Morrow J A, Collie I T, Dunbar D R, Walker G B, Shahid M, Hill D R
Target Discovery Section, Organon Laboratories Limited, Newhouse, Lanarkshire, UK.
FEBS Lett. 1998 Nov 20;439(3):334-40. doi: 10.1016/s0014-5793(98)01390-8.
Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We have cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na+/Cl--dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose-dependent uptake of glycine with an apparent Km of 108 microM. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD=8.988) on human chromosome 11p15.1-15.2.
神经递质转运系统是突触功能治疗性改变的主要靶点。我们已经从人脑和脊髓中克隆并测序了编码人2型甘氨酸转运体GlyT2的cDNA。一个2391个核苷酸的开放阅读框编码一个797个氨基酸的蛋白质，该蛋白质以Na⁺/Cl⁻依赖的方式转运甘氨酸。当在CHO细胞中稳定表达时，人GlyT2表现出对甘氨酸的剂量依赖性摄取，表观Km为108微摩尔。在浓度高达1毫摩尔时，肌氨酸对这种摄取没有影响。辐射杂交分析将GlyT2基因定位到人类染色体11p15.1 - 15.2上的D11S1308（LOD = 8.988）。
