Sato H, Iwano M, Akai Y, Kurioka H, Kubo A, Yamaguchi T, Hirata E, Kanauchi M, Dohi K
1st Department of Internal Medicine, Nara Medical University, Nara, Japan.
Am J Nephrol. 1998;18(6):490-4. doi: 10.1159/000013415.
The accumulation of extracellular matrix in the glomeruli of human and experimental models of diabetic nephropathy is associated with disease progression. Transforming growth factor beta 1 (TGF-beta1), which is a multifunctional peptide growth factor, plays a key role in the synthesis of extracellular matrix protein in vitro, and the expression of TGF-beta1 is elevated in human and rat diabetic nephropathy. In this study, we measured the urinary TGF-beta1 excretion in 57 patients with non-insulin-dependent diabetes mellitus and in 20 healthy volunteers to examine whether the determination of urinary TGF-beta1 excretion would facilitate the evaluation of the degree of mesangial expansion in patients with diabetic nephropathy. Both active and total TGF-beta1 levels in 24-hour urine samples collected from patients with diabetes mellitus and normal controls were measured using an enzyme-linked immunosorbent assay. We observed a higher excretion of urinary TGF-beta1 in patients with diabetes mellitus than in normal controls. In addition, the urinary TGF-beta1 excretion was elevated in patients with severe mesangial expansion. These results suggest that urinary TGF-beta1 may represent one parameter that can be used to evaluate the progression of diabetic nephropathy.
在人类糖尿病肾病及实验模型的肾小球中，细胞外基质的积聚与疾病进展相关。转化生长因子β1（TGF-β1）是一种多功能肽生长因子，在体外细胞外基质蛋白合成中起关键作用，且在人类和大鼠糖尿病肾病中TGF-β1的表达升高。在本研究中，我们检测了57例非胰岛素依赖型糖尿病患者及20名健康志愿者的尿TGF-β1排泄量，以探讨测定尿TGF-β1排泄量是否有助于评估糖尿病肾病患者的系膜扩张程度。采用酶联免疫吸附测定法检测糖尿病患者和正常对照者24小时尿液样本中的活性TGF-β1和总TGF-β1水平。我们观察到糖尿病患者尿TGF-β1排泄量高于正常对照者。此外，重度系膜扩张患者的尿TGF-β1排泄量升高。这些结果表明，尿TGF-β1可能是可用于评估糖尿病肾病进展的一个参数。
