Fujisawa K, Itoh K, Imai Y, Itoh H, Yamamoto M
Department of Pathology, Kobe University School of Medicine, Japan.
Kobe J Med Sci. 1998 Feb;44(1):19-30.
We studied 30 cases of retinoblastoma (Rb) pathologically and histochemically. Rb cases were classified into two types, 18 cases of differentiated (D-type) and 12 undifferentiated type (U-type) according to International Classification of Diseases for Oncology(1990, WHO). D-type of Rb is found more commonly in younger infants less than 1 year, but U-type found more frequently in aged infants from 2 to 4 years. D-type Rb showed more marked necrotic areas, degeneration and calcification. Immunohistochemical stains were carried out in 21 cases of Rb by use of MIB-1, PCNA, bcl-2, Fas, BAX, NF, SP and Tunel stains, respectively. MIB-1 is a good marker of proliferation of Rb cells and its high positivity correlates closely to more extensive necrotic areas of Rb cases. There are only a few positive cells by Tunel stain suggesting apoptotic cells, but diffuse positive cells by bcl-2 and Fas and completely negative by BAX gene. From these results, it can be seen that differentiation of Rb cells may be affected by bcl-2 and Fas but not by BAX gene.
我们对30例视网膜母细胞瘤（Rb）进行了病理和组织化学研究。根据世界卫生组织《国际肿瘤疾病分类》（1990年），Rb病例分为两种类型，18例分化型（D型）和12例未分化型（U型）。D型Rb在1岁以下的婴儿中更为常见，而U型在2至4岁的大龄婴儿中更为常见。D型Rb显示出更明显的坏死区域、变性和钙化。分别使用MIB-1、PCNA、bcl-2、Fas、BAX、NF、SP和Tunel染色对21例Rb进行了免疫组织化学染色。MIB-1是Rb细胞增殖的良好标志物，其高阳性率与Rb病例更广泛的坏死区域密切相关。Tunel染色仅有少数阳性细胞提示凋亡细胞，但bcl-2和Fas染色呈弥漫阳性，BAX基因染色完全阴性。从这些结果可以看出，Rb细胞的分化可能受bcl-2和Fas影响，而不受BAX基因影响。
