van Baare J, Cameron P U, Vardaxis N, Pagnon J, Reece J, Middelkoop E, Crowe S M
Euro Skin Bank, Beverwijk, The Netherlands.
J Burn Care Rehabil. 1998 Nov-Dec;19(6):494-500. doi: 10.1097/00004630-199811000-00007.
Cryopreservation and glycerol preservation are 2 successful methods for long-term preservation of human cadaver skin. Preservation is subjected to strict criteria to minimize the risk of disease transmission. This investigation compares the effects of glycerol preservation and cryopreservation on the inactivation of HIV-1. The effects of glycerol preservation and cryopreservation on inactivation of both extracellular and intracellular HIV-1Ba-L were investigated. After exposing HIV-1Ba-L-infected material to various concentrations of glycerol or to 10% dimethyl sulfoxide followed by cryopreservation, uninfected peripheral blood mononuclear cells were added to the treated material. At different time points during the culture, supernatants were taken to quantify HIV-1Ba-L and reverse transcriptase levels to determine HIV-1Ba-L infectivity. Cell-free HIV-1Ba-L was inactivated within 30 minutes in 70% and 85% glycerol. Also, intracellular HIV-1Ba-L in infected peripheral blood mononuclear cells or infected cadaver skin was completely inactivated by glycerol treatment in vitro. Cryopreservation did not show any extracellular or intracellular HIV-1Ba-L inactivation. Glycerol preservation--but not cryopreservation--of human cadaveric donor skin can inactivate both extracellular and intracellular HIV-1.
冷冻保存和甘油保存是长期保存人尸体皮肤的两种成功方法。保存需遵循严格标准，以将疾病传播风险降至最低。本研究比较了甘油保存和冷冻保存对HIV-1灭活的影响。研究了甘油保存和冷冻保存对细胞外和细胞内HIV-1Ba-L灭活的影响。将感染HIV-1Ba-L的材料暴露于不同浓度的甘油或10%二甲基亚砜后进行冷冻保存，然后向处理过的材料中加入未感染的外周血单核细胞。在培养过程中的不同时间点，取上清液定量HIV-1Ba-L和逆转录酶水平，以确定HIV-1Ba-L的感染性。游离的HIV-1Ba-L在70%和85%的甘油中30分钟内被灭活。此外，感染的外周血单核细胞或感染的尸体皮肤中的细胞内HIV-1Ba-L在体外经甘油处理后被完全灭活。冷冻保存未显示出对细胞外或细胞内HIV-1Ba-L的任何灭活作用。人尸体供皮的甘油保存（而非冷冻保存）可灭活细胞外和细胞内的HIV-1。
