凝血酶和抗凝血酶III在生物特异性硅色层上的色谱分析
[Chromatography of thrombin and antithrombin III on biospecific silochromes].
作者信息
Lohins'ka Z V, Oryshchenko N V, Vus M M, Maherovskyĭ Iu V, Haĭda H Z, Haĭda A V
机构信息
Lviv Branch of Kyiev Research Institute of Haematology and Blood Transfusion.
出版信息
Ukr Biokhim Zh (1978). 1998 May-Jun;70(3):63-8.
It was shown that the seaweed sulfopolysaccharides Furcellaria and Phyllophora--furcellaran and phyllophoran--are the competitive inhibitors of thrombin-induced fibrinogen clotting. Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Possible application of the synthesized adsorbents both for thrombin, AT III purification from blood products and for alpha-thrombin, beta and gamma-thrombin separation are discussed.
结果表明，海藻硫酸多糖角叉菜属和叶状藻属——角叉菜聚糖和叶状藻聚糖——是凝血酶诱导纤维蛋白原凝血的竞争性抑制剂。以肝素、叶状藻聚糖和角叉菜聚糖为配体，在氨丙基硅铬上合成了用于分离凝血酶和抗凝血酶III（AT III）的生物特异性吸附剂，并研究了这些蛋白质在该吸附剂上相对于肝素纤维素和肝素琼脂糖的吸附特性。讨论了合成吸附剂在从血液制品中纯化凝血酶、AT III以及分离α-凝血酶、β-凝血酶和γ-凝血酶方面的可能应用。
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