O'Bryan M K, Zini A, Cheng C Y, Schlegel P N
The Population Council, Center for Biomedical Research, New York, New York, USA.
Fertil Steril. 1998 Dec;70(6):1143-7. doi: 10.1016/s0015-0282(98)00382-3.
To characterize the pattern of endothelial nitric oxide synthase (eNOS) expression on human spermatozoa and to determine whether sperm eNOS expression correlates with sperm function.
Prospective, observational study.
University infertility clinic.
PATIENT(S): Twelve nonazoospermic infertile men.
INTERVENTION(S): Semen samples (n=12) obtained from nonazoospermic infertile men were fractionated on discontinuous Percoll gradients. Endothelial nitric oxide synthase staining on spermatozoa was correlated with sperm motility in Percoll gradient-fractionated spermatozoa. Endothelial nitric oxide synthase protein was detected with the use of a previously characterized monoclonal antibody. Control slides were incubated with preabsorbed antibody or mouse immunoglobulin G.
MAIN OUTCOME MEASURE(S): Localization of eNOS on human spermatozoa and correlation between the pattern of sperm eNOS expression and sperm motility.
RESULT(S): Morphologically normal spermatozoa exhibited postacrosomal and equatorial eNOS immunostaining. However, abnormally shaped spermatozoa often exhibited aberrant staining (in the midpiece and/or head region). A significant negative correlation was observed between the percentage of sperm with aberrant eNOS immunostaining and the percentage of motile sperm (r=-.46).
CONCLUSION(S): The specific localization of eNOS to human spermatozoa suggests that nitric oxide may be involved in normal sperm physiology. However, aberrant patterns of sperm eNOS expression are associated with decreased sperm motility, possibly through the generation of excessive cytotoxic oxidants.
描述人精子内皮型一氧化氮合酶（eNOS）的表达模式，并确定精子eNOS表达是否与精子功能相关。
前瞻性观察研究。
大学不孕不育诊所。
12名非无精子症的不育男性。
从非无精子症的不育男性获取精液样本（n = 12），通过不连续的Percoll梯度进行分离。对Percoll梯度分离的精子进行内皮型一氧化氮合酶染色，并将其与精子活力相关联。使用先前鉴定的单克隆抗体检测内皮型一氧化氮合酶蛋白。对照玻片用预吸附抗体或小鼠免疫球蛋白G孵育。
eNOS在人精子上的定位以及精子eNOS表达模式与精子活力之间的相关性。
形态正常的精子在顶体后和赤道部位呈现eNOS免疫染色。然而，形状异常的精子通常呈现异常染色（在中段和/或头部区域）。观察到eNOS免疫染色异常的精子百分比与活动精子百分比之间存在显著负相关（r = -0.46）。
eNOS在人精子上的特定定位表明一氧化氮可能参与正常精子生理功能。然而，精子eNOS表达异常模式与精子活力降低相关，可能是通过产生过量的细胞毒性氧化剂所致。
