Wyszynski M, Kim E, Yang F C, Sheng M
Howard Hughes Medical Institute, Department of Neurobiology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Neuropharmacology. 1998 Oct-Nov;37(10-11):1335-44. doi: 10.1016/s0028-3908(98)00120-8.
The mechanisms by which glutamate receptors are concentrated in brain excitatory synapses are believed to involve interactions between receptor subunits and postsynaptic anchoring or scaffolding proteins. Recently GRIP, a protein containing seven PDZ domains, was identified as an AMPA receptor binding protein and implicated in the synaptic targeting of AMPA receptors. Here we show that GRIP mRNA is also expressed in some tissues outside of the brain, including testis and kidney. Specific antibodies were raised to study GRIP protein. On Western blots, GRIP protein appears as a heterogeneous band (approximately 130 kilodaltons) which is expressed in widespread brain regions and throughout postnatal development. Biochemical studies reveal that GRIP is largely membrane associated and enriched in the postsynaptic density (PSD), though not as highly concentrated in the PSD as is PSD-95. By immunohistochemistry, GRIP is distributed in a somatodendritic pattern in neurons of adult rat brain, with especially prominent expression in a subset of interneurons.
谷氨酸受体在脑兴奋性突触中聚集的机制被认为涉及受体亚基与突触后锚定或支架蛋白之间的相互作用。最近，GRIP（一种含有7个PDZ结构域的蛋白质）被鉴定为AMPA受体结合蛋白，并与AMPA受体的突触靶向有关。在这里，我们表明GRIP mRNA也在脑外的一些组织中表达，包括睾丸和肾脏。制备了特异性抗体来研究GRIP蛋白。在蛋白质免疫印迹法中，GRIP蛋白表现为一条异质性条带（约130千道尔顿），在广泛的脑区以及整个出生后发育过程中均有表达。生化研究表明，GRIP主要与膜相关，并在突触后致密区（PSD）中富集，尽管其在PSD中的浓度不如PSD - 95高。通过免疫组织化学方法，GRIP以躯体树突状模式分布在成年大鼠脑神经元中，在一部分中间神经元中表达尤为突出。
