呫吨醇/尿素法中试剂变异性来源的鉴定
Identification of the source of reagent variability in the xanthydrol/urea method.
作者信息
Biles P V, Ziobro G C
机构信息
U.S. Food and Drug Administration, Division of Natural Products, Washington, DC 20204, USA.
出版信息
J AOAC Int. 1998 Nov-Dec;81(6):1155-61.
Contamination of food and food packaging material by rodent urine is evidence of insanitary conditions. Urea from rodent urine is used as a chemical indicator of contamination. The limit of detection of the xanthydrol/urea AOAC Method 959.14 by formation of dixanthylurea crystals is 4 micrograms urea isolated from urine on packaging material. Six different lots of xanthydrol from 5 different manufacturers were compared. Differences in urea detection sensitivity of the xanthydrol of up to 1000-fold were observed. Melting points showed further evidence of variability and impurities in xanthydrol lots. A liquid chromatographic method was developed to separate and identify the impurities. Confirmation of analytes was performed by gas chromatography/mass spectrometry.
啮齿动物尿液对食品及食品包装材料的污染是卫生条件差的证据。啮齿动物尿液中的尿素被用作污染的化学指示剂。通过形成二黄原脲晶体的黄嘌呤醇/尿素AOAC方法959.14的检测限为从包装材料上的尿液中分离出的4微克尿素。比较了来自5个不同制造商的6个不同批次的黄嘌呤醇。观察到黄嘌呤醇的尿素检测灵敏度差异高达1000倍。熔点进一步显示了黄嘌呤醇批次的变异性和杂质。开发了一种液相色谱方法来分离和鉴定杂质。通过气相色谱/质谱法对分析物进行确证。
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