Busque P, Higgins R, Sénéchal S, Marchand R, Quessy S
Department of Pathology and Microbiology, College of Veterinary Medicine, University of Montreal, Quebec, Canada.
Vet Microbiol. 1998 Oct;63(2-4):229-38. doi: 10.1016/s0378-1135(98)00216-8.
A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60 min of incubation, 80.2 +/- 2.8% of swine granulocytes and 77.0 +/- 2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5 +/- 3.2% of human granulocytes and in 92.8 +/- 3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different.
采用一种简单的流式细胞术方法，同时研究猪和人的多形核血白细胞及单核血白细胞对2型猪链球菌的吞噬作用。使用该方法，细菌与白细胞比例为10:1，孵育60分钟后，结果显示80.2±2.8%的猪粒细胞和77.0±2.8%的猪单核细胞含有异硫氰酸荧光素（FITC）标记的2型猪链球菌735菌株。使用同一菌株，在95.5±3.2%的人粒细胞和92.8±3.6%的人单核细胞中发现了FITC标记的细菌。猪链球菌无毒株和有毒株的吞噬率无显著差异。
