通过多形核和单核血液白细胞同时进行流式细胞术测量猪链球菌的吞噬作用。
Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes.
作者信息
Busque P, Higgins R, Sénéchal S, Marchand R, Quessy S
机构信息
Department of Pathology and Microbiology, College of Veterinary Medicine, University of Montreal, Quebec, Canada.
出版信息
Vet Microbiol. 1998 Oct;63(2-4):229-38. doi: 10.1016/s0378-1135(98)00216-8.
A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60 min of incubation, 80.2 +/- 2.8% of swine granulocytes and 77.0 +/- 2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5 +/- 3.2% of human granulocytes and in 92.8 +/- 3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different.
采用一种简单的流式细胞术方法，同时研究猪和人的多形核血白细胞及单核血白细胞对2型猪链球菌的吞噬作用。使用该方法，细菌与白细胞比例为10:1，孵育60分钟后，结果显示80.2±2.8%的猪粒细胞和77.0±2.8%的猪单核细胞含有异硫氰酸荧光素（FITC）标记的2型猪链球菌735菌株。使用同一菌株，在95.5±3.2%的人粒细胞和92.8±3.6%的人单核细胞中发现了FITC标记的细菌。猪链球菌无毒株和有毒株的吞噬率无显著差异。
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