Adams V, Krabbes S, Jiang H, Yu J, Rahmel A, Gielen S, Schuler G, Hambrecht R
Heart Center GmbH, Division of Cardiology, University of Leipzig, Germany.
Nitric Oxide. 1998;2(4):242-9. doi: 10.1006/niox.1998.0184.
The presence of the inducible nitric oxide synthase (iNOS) has previously been reported in heart and skeletal muscle of patients with chronic heart failure, where it is thought to be involved in the pathogenesis of dilated cardiomyopathy or of exercise intolerance observed in patients with chronic heart failure. To identify the iNOS of these human tissues the cDNA sequence was determined using reverse transcription polymerase chain reaction (RT-PCR). The deduced amino acid sequence of human heart and skeletal muscle shows a 99% identity between each other. The amino acid sequence was distinct from the constitutively expressed nitric oxide synthases. A 98% identity at the protein level was detected to rat vascular smooth muscle iNOS, whereas the homology to human hepatocyte iNOS was only 79%. The cofactor binding sites in the reported iNOS from human heart and skeletal muscle are highly conserved. The iNOS sequence described in this report is the first one isolated directly from human tissue expressing iNOS in the circumstances of chronic heart failure.
此前有报道称，慢性心力衰竭患者的心脏和骨骼肌中存在诱导型一氧化氮合酶（iNOS），据认为它参与了慢性心力衰竭患者中观察到的扩张型心肌病或运动不耐受的发病机制。为了鉴定这些人体组织中的iNOS，使用逆转录聚合酶链反应（RT-PCR）测定了cDNA序列。推导的人心脏和骨骼肌氨基酸序列彼此间显示出99%的同一性。该氨基酸序列与组成型表达的一氧化氮合酶不同。在蛋白质水平上检测到与人心脏和骨骼肌iNOS与大鼠血管平滑肌iNOS有98%的同一性，而与人肝细胞iNOS的同源性仅为79%。在报道的人心脏和骨骼肌iNOS中的辅因子结合位点高度保守。本报告中描述的iNOS序列是首次在慢性心力衰竭情况下直接从表达iNOS的人体组织中分离得到的。
