Shinozaki K, Maruyama K, Kume H, Tomita T, Saido T C, Iwatsubo T, Obata K
Laboratory of Neurochemistry, National Institute for Physiological Sciences, Myodaiji, Okazaki, Aichi 444, Japan.
Int J Mol Med. 1998 May;1(5):797-9. doi: 10.3892/ijmm.1.5.797.
Presenilin 2 (PS2) is a gene responsible for the early-onset familial Alzheimer's disease (AD). PS2 mutations are considered to be closely related to the pathogenesis of AD. We screened for proteins that interact with PS2 to understand its pathological and physiological functions. Using the PS2 loop domain as the bait, the yeast two-hybrid system was used for screening, and mu-calpain was identified as a PS2 binding protein. In COS-1 cells, the interaction of PS2 with mu-calpain was confirmed by immunoprecipitation. These results suggested that PS2 and mu-calpain interact with each other, and might regulate each other's functions.
早老素2（PS2）是一种与早发性家族性阿尔茨海默病（AD）相关的基因。PS2突变被认为与AD的发病机制密切相关。我们筛选了与PS2相互作用的蛋白质，以了解其病理和生理功能。以PS2环结构域为诱饵，利用酵母双杂交系统进行筛选，鉴定出μ-钙蛋白酶为PS2结合蛋白。在COS-1细胞中，通过免疫沉淀证实了PS2与μ-钙蛋白酶的相互作用。这些结果表明，PS2与μ-钙蛋白酶相互作用，并可能相互调节彼此的功能。
