Oshima K H, Evans-Strickfaden T, Highsmith A
Biological Products Branch, Scientific Resources Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, Atlanta, GA, USA.
Vox Sang. 1998;75(3):181-8.
We examined the ability of a modified polyvinylidene fluoride membrane filter to remove blood-borne and surrogate viruses.
Phages PR772 and PP7, hepatitis B virus (HBV), hepatitis C virus and simian virus 40 (SV40) were spiked in minimum essential media with 10% fetal calf serum and the concentration of these viruses compared before and after filtration by either plaque assay or polymerase chain reaction.
Viruses >50 nm were removed to below detection limits (>10(6) logs) for all filters tested. A 5-log reduction of HBV (42 nm) and 2- to 3-log reduction of HCV (30-65 nm) was observed.
A predictable size-based removal of viral agents was observed. The results also suggest the possible utility of SV40 as a surrogate to HBV for membrane filter challenge studies.
我们检测了一种改良的聚偏二氟乙烯膜过滤器去除血源性病原体和替代病毒的能力。
将噬菌体PR772和PP7、乙型肝炎病毒（HBV）、丙型肝炎病毒和猴病毒40（SV40）加入含有10%胎牛血清的最低必需培养基中，通过噬斑测定法或聚合酶链反应比较过滤前后这些病毒的浓度。
对于所有测试的过滤器，直径大于50 nm的病毒均被去除至检测限以下（大于10⁶对数）。观察到HBV（42 nm）减少了5个对数，HCV（30 - 65 nm）减少了2至3个对数。
观察到基于大小的病毒制剂可预测去除效果。结果还表明SV40作为HBV的替代物用于膜过滤器挑战性研究的潜在用途。
