大鼠背根神经节中潜伏性水痘-带状疱疹病毒的证据。

Evidence of latent varicella-zoster virus in rat dorsal root ganglia.

作者信息

Annunziato P, LaRussa P, Lee P, Steinberg S, Lungu O, Gershon A A, Silverstein S

机构信息

Department of Pediatrics, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.

出版信息

J Infect Dis. 1998 Nov;178 Suppl 1:S48-51. doi: 10.1086/514261.

DOI:10.1086/514261

PMID:9852973

Abstract

Latent varicella-zoster virus (VZV) was studied in ganglia of rats that had been inoculated subcutaneously with either a high-passaged wild-type, a low-passaged wild-type, or the vaccine strain of virus using in situ hybridization. Nine of 11 rats injected with virus and no control rats developed serum VZV antibodies as demonstrated by fluorescent antibody membrane antigen. Polymerase chain reaction 2 weeks following inoculation did not detect viremia in the rats. VZV was detected by in situ hybridization in ganglia of 10 of the 11 infected rats but not in ganglia of the control rats. The distribution of VZV DNA is identical to that seen in humans; satellite cells and neurons contain VZV DNA. Although all animals received unilateral injections of virus, VZV DNA was in ipsilateral and contralateral ganglia in 6 animals, suggesting that virus replication and viremia had occurred.

摘要

使用原位杂交技术，对皮下接种了高传代野生型、低传代野生型或病毒疫苗株的大鼠神经节中的潜伏水痘带状疱疹病毒（VZV）进行了研究。通过荧光抗体膜抗原检测，11只注射病毒的大鼠中有9只产生了血清VZV抗体，而对照大鼠未产生。接种后2周的聚合酶链反应未在大鼠中检测到病毒血症。通过原位杂交在11只感染大鼠中的10只的神经节中检测到了VZV，但在对照大鼠的神经节中未检测到。VZV DNA的分布与在人类中观察到的相同；卫星细胞和神经元含有VZV DNA。尽管所有动物均接受了单侧病毒注射，但6只动物的同侧和对侧神经节中均存在VZV DNA，这表明发生了病毒复制和病毒血症。