Matsumoto S, Takahashi M, Kitadai N, Katae H
Research Laboratories, Animal Science Division, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
J Vet Med Sci. 1998 Nov;60(11):1259-61. doi: 10.1292/jvms.60.1259.
Urine samples of cats and dogs collected for 24 hr after a subcutaneous injection of orbifloxacin (OBFX) were analyzed. The metabolites were examined using HPLC. In the dog urine, 87% of total was the parent compound and 13% glucuronide compound of OBFX and 96% was parent and 4% metabolite in the cat urine. The metabolite of cat urine was identified as N-hydroxy OBFX, determined by comparison of the extraction of urine with chloroform with the standard compound of N-hydroxy OBFX, using LC/APCIMS. N-hydroxy OBFX had a weaker antibacterial activity against fluoroquinolone sensitive bacteria than the parent compound.
对皮下注射奥比沙星（OBFX）后24小时收集的猫和狗的尿液样本进行了分析。使用高效液相色谱法检测代谢产物。在狗尿液中，总量的87%为母体化合物，13%为OBFX的葡萄糖醛酸化合物；在猫尿液中，96%为母体，4%为代谢产物。通过将尿液用氯仿萃取物与N-羟基OBFX标准化合物进行比较，利用液相色谱/大气压化学电离质谱法鉴定出猫尿液中的代谢产物为N-羟基OBFX。N-羟基OBFX对氟喹诺酮敏感菌的抗菌活性比母体化合物弱。
