Endo T, Tamaki K, Arimura Y, Itoh F, Hinoda Y, Hareyama M, Irimura T, Fujita M, Imai K
First Department of Internal Medicine, Sapporo Medical University, Japan.
J Gastroenterol. 1998 Dec;33(6):811-5. doi: 10.1007/s005350050180.
Columnar epithelium-lined esophagus (Barrett's esophagus) is an acquired disorder associated with a high incidence of adenocarcinoma of the lower esophagus. Columnar epithelium resembling intestinal metaplasia (IM) is especially important, since it is considered to be a premalignant condition. The aim of this study was to define the sulfated carbohydrate chain of mucin and its core peptide profile in Barrett's esophagus (BE) and to compare it with the profile in Barrett's adenocarcinoma and lower esophageal adenocarcinoma. The sulfated carbohydrate chain was not expressed in 16 specimens of normal esophageal epithelium, but in BE, it was expressed in 50% (8/16) of the specimens. This chain was detected in 100% (7/7) of esophageal adenocarcinoma specimens, including four cases of Barrett's adenocarcinoma. These data suggest that the sulfated carbohydrate chain may be associated with malignant phenotype of the esophagus. MUC1 core peptide was positive in 87% (13/15) of BE specimens and in 29% (2/7) of the esophageal adenocarcinoma specimens. MUC2 core peptide was present in 57% (8/14) and 43% (3/7) of these specimens, respectively. These data suggest that Barrett's epithelium, which is similar to IM, but not normal esophageal epithelium, expresses the sulfated sugar chain which is known to be present in gastric IM and colonic mucosa. However, there was no significant correlation between the expression of the sulfated sugar chain and the expression of either mucin core peptide MUC1 or MUC2. Thus, this carbohydrate chain may be expressed on as yet unidentified core proteins, other than MUC1 or MUC2 core peptide, in BE and esophageal adenocarcinoma. Identification of these proteins may be very important in helping to detect premalignant status in BE.
柱状上皮化生食管（巴雷特食管）是一种后天性疾病，与下食管腺癌的高发病率相关。类似肠化生（IM）的柱状上皮尤为重要，因为它被认为是一种癌前病变。本研究的目的是确定巴雷特食管（BE）中黏蛋白的硫酸化碳水化合物链及其核心肽谱，并将其与巴雷特腺癌和下食管腺癌的谱进行比较。在16例正常食管上皮标本中未检测到硫酸化碳水化合物链，但在BE中，50%（8/16）的标本中表达了该链。在100%（7/7）的食管腺癌标本中检测到了该链，其中包括4例巴雷特腺癌。这些数据表明硫酸化碳水化合物链可能与食管的恶性表型有关。MUC1核心肽在87%（13/15）的BE标本和29%（2/7）的食管腺癌标本中呈阳性。MUC2核心肽分别存在于57%（8/14）和43%（3/7）的这些标本中。这些数据表明，与IM相似但不同于正常食管上皮的巴雷特上皮表达了已知存在于胃IM和结肠黏膜中的硫酸化糖链。然而，硫酸化糖链的表达与黏蛋白核心肽MUC1或MUC2的表达之间没有显著相关性。因此，在BE和食管腺癌中，这种碳水化合物链可能在除MUC1或MUC2核心肽之外尚未鉴定的核心蛋白上表达。鉴定这些蛋白对于帮助检测BE中的癌前状态可能非常重要。
