Rao A M, Hatcher J F, Baskaya M K, Dempsey R J
Department of Neurological Surgery, Clinical Science Center, University of Wisconsin-Madison, 53792-3232, USA.
Neurosci Lett. 1998 Nov 6;256(2):65-8. doi: 10.1016/s0304-3940(98)00780-0.
Ornithine decarboxylase (ODC) is considered the rate-limiting enzyme in polyamine biosynthesis. An increase in putrescine (a natural polyamine) synthesis after central nervous system (CNS) injury appears to be involved in blood-brain barrier dysfunction, development of vasogenic edema and neuronal death. An improved method is described to determine the ODC activity as well as polyamine levels from the same brain tissue. The polyamine results showed no significant differences from data obtained with the conventional assay. The advantages of this method are to: (1) minimize the number of animals needed for the study, and (2) eliminate any internal inconsistencies resulting from use of two independent groups of animals for ODC and polyamine measurements. Using this method, ODC activities and polyamine levels were measured in cortices and hippocampi from global transient ischemia of gerbils and traumatic brain injury (TBI) of rats.
鸟氨酸脱羧酶（ODC）被认为是多胺生物合成中的限速酶。中枢神经系统（CNS）损伤后腐胺（一种天然多胺）合成的增加似乎与血脑屏障功能障碍、血管源性水肿的发展和神经元死亡有关。本文描述了一种改进的方法，可从同一脑组织中测定ODC活性以及多胺水平。多胺结果与传统检测方法获得的数据无显著差异。该方法的优点是：（1）减少研究所需动物的数量，（2）消除因使用两组独立动物进行ODC和多胺测量而产生的任何内部不一致性。使用该方法，在沙土鼠全脑短暂性缺血和大鼠创伤性脑损伤（TBI）后的皮质和海马中测量了ODC活性和多胺水平。
