Seven A, Taşan E, Inci F, Hatemi H, Burçak G
Department of Biochemistry, Cerrahpaşa Medical Faculty, Istanbul University, Turkey.
Clin Chem Lab Med. 1998 Oct;36(10):767-70. doi: 10.1515/CCLM.1998.136.
In this study the impact of vitamin C supplementation on oxidative damage as assessed by thiobarbituric acid reactive substances and markers of antioxidant status: namely Cu/Zn superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione were investigated in 24 hyperthyroid patients under propylthiouracil therapy (3x100 mg/day) for five days and in 15 healthy controls. Ascorbic acid (1000 mg/day) was given as a supplement for 1 month to both the patients and controls during the study period. Heparinised blood samples were taken at the beginning and the end of one month ascorbic acid supplementation. Comparison of the hyperthyroid patients with the controls revealed higher lipid peroxidation (p<0.001), higher Cu/Zn superoxide dismutase activity (p<0.001), higher glutathione level (p<0.001) and lower glutathione reductase activity (p<0.001). Vitamin C supplementation to hyperthyroid patients caused significant increases in glutathione concentration (p<0.001) and glutathione peroxidase activity (p<0.001), whereas there were significant decreases in glutathione reductase (p<0.001) and Cu/Zn superoxide dismutase activities (p<0.01). Thiobarbituric acid reactive substances and thiobarbituric acid reactive substances/glutathione ratio were significantly decreased (p<0.01). Vitamin C supplementation to euthyroid controls caused significant increases in glutathione concentration (p<0.001) and glutathione peroxidase and Cu/Zn superoxide dismutase activities (p<0.001), whereas there was a significant decrease in glutathione reductase (p<0.001). The thiobarbituric acid reactive substances/glutathione ratio was significantly decreased (p<0.05). Our findings reveal the potentiation of antioxidant status and a relief in oxidative stress in both propylthiouracil treated hyperthyroid patients and controls in response to vitamin C supplementation.
在本研究中，对24例接受丙硫氧嘧啶治疗（3×100mg/天）5天的甲状腺功能亢进患者及15名健康对照者，研究了补充维生素C对通过硫代巴比妥酸反应性物质评估的氧化损伤以及抗氧化状态标志物（即铜/锌超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽）的影响。在研究期间，患者和对照者均补充抗坏血酸（1000mg/天）1个月。在补充抗坏血酸1个月的开始和结束时采集肝素化血样。甲状腺功能亢进患者与对照者的比较显示，脂质过氧化水平更高（p<0.001）、铜/锌超氧化物歧化酶活性更高（p<0.001）、谷胱甘肽水平更高（p<0.001）以及谷胱甘肽还原酶活性更低（p<0.001）。对甲状腺功能亢进患者补充维生素C导致谷胱甘肽浓度显著升高（p<0.001）和谷胱甘肽过氧化物酶活性显著升高（p<0.001），而谷胱甘肽还原酶（p<0.001）和铜/锌超氧化物歧化酶活性显著降低（p<0.01）。硫代巴比妥酸反应性物质及硫代巴比妥酸反应性物质/谷胱甘肽比值显著降低（p<0.01）。对甲状腺功能正常的对照者补充维生素C导致谷胱甘肽浓度显著升高（p<0.001）以及谷胱甘肽过氧化物酶和铜/锌超氧化物歧化酶活性显著升高（p<0.001），而谷胱甘肽还原酶显著降低（p<0.001）。硫代巴比妥酸反应性物质/谷胱甘肽比值显著降低（p<0.05）。我们的研究结果表明，补充维生素C后，丙硫氧嘧啶治疗的甲状腺功能亢进患者和对照者的抗氧化状态均得到增强，氧化应激得到缓解。
