微囊藻毒素-LR对人RSa细胞的致突变性。

Mutagenicity of microcystin-LR in human RSa cells.

作者信息

Suzuki H, Watanabe M F, Wu Y, Sugita T, Kita K, Sato T, Wang X, Tanzawa H, Sekiya S, Suzuki N

机构信息

Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.

出版信息

Int J Mol Med. 1998 Jul;2(1):109-12. doi: 10.3892/ijmm.2.1.109.

DOI:10.3892/ijmm.2.1.109

PMID:9854153

Abstract

Microcystin-LR (MCLR) is a potent cyclic heptapeptidic hepatotoxin produced by bloom-forming cyanobacteria. The mutagenicity induced by this compound in cultured human RSa cells was found by determination of ouabain-resistant (OuaR) mutation, with the highest frequency at the concentration of 15 microgram/ml. Moreover, base substitution mutations at K-ras codon 12 in genomic DNA, assessed by polymerase chain reaction (PCR) and differential dot-blot hybridization using digoxigenin-labeled probes, were detected in RSa cells 6 days after exposure to MCLR (7.5-15 microgram/ml).

摘要

微囊藻毒素-LR（MCLR）是一种由形成水华的蓝藻产生的强效环状七肽肝毒素。通过测定哇巴因抗性（OuaR）突变发现，该化合物在培养的人RSa细胞中诱导的致突变性，在浓度为15微克/毫升时频率最高。此外，在暴露于MCLR（7.5 - 15微克/毫升）6天后的RSa细胞中，通过聚合酶链反应（PCR）和使用地高辛标记探针的差异点杂交评估，检测到基因组DNA中K-ras密码子12处的碱基替换突变。