Pitt-Rivers R
Biochem J. 1976 Sep 1;157(3):767-8. doi: 10.1042/bj1570767.
Human and rat thyroglobulin were reduced and alkylated in aqueous alkaline conditions in the absence of denaturants; the product of reduction in both cases has been found to have mol.wt. about 165000, or one-quarter that of the native molecule.
在无变性剂存在的情况下,将人及大鼠甲状腺球蛋白在碱性水溶液中进行还原和烷基化处理;已发现两种情况下还原产物的分子量约为165000,即天然分子的四分之一。