角豆荚：黑曲霉生产柠檬酸的新型底物。

Carob pod: a new substrate for citric acid production by Aspergillus niger.

作者信息

Roukas T

机构信息

Department of Food Science and Technology, Aristotle University of Thessaloniki, Greece.

出版信息

Appl Biochem Biotechnol. 1998 Jul;74(1):43-53. doi: 10.1007/BF02786885.

DOI:10.1007/BF02786885

PMID:9854802

Abstract

The production of citric acid from carob pod extract by A. niger in surface fermentation was investigated. A maximum citric acid concentration (85.5 g/L), citric acid productivity (4.07 g/L/d), specific citric acid production rate (0.18 g/g/d), and specific sugar uptake rate (0.358 g/g/d) was achieved at an initial sugar concentration of 200 g/L, pH of 6.5, and a temperature of 30 degrees C. Other kinetic parameters, namely, citric acid yield, biomass yield, specific biomass production rate, and fermentation efficiency were maximum at pH 6.5, temperature 30 degrees C, and initial sugar concentration 100 g/L. The external addition of methanol into the carob pod extract at a concentration up to 4% (v/v) improved the production of citric acid.

摘要

研究了黑曲霉在表面发酵中利用角豆荚提取物生产柠檬酸的情况。当初始糖浓度为200 g/L、pH值为6.5、温度为30℃时，柠檬酸浓度达到最大值（85.5 g/L），柠檬酸生产率为（4.07 g/L/d），比柠檬酸生产速率为（0.18 g/g/d），比糖摄取速率为（0.358 g/g/d）。其他动力学参数，即柠檬酸产率、生物量产率、比生物量生产速率和发酵效率，在pH值6.5、温度30℃和初始糖浓度100 g/L时达到最大值。向角豆荚提取物中额外添加浓度高达4%（v/v）的甲醇可提高柠檬酸产量。