Pang D, Popescu G, Rodgers J, Berman B L, Dritschilo A
Department of Radiation Medicine, Georgetown University Medical Center, Washington, DC 20007, USA.
Scanning Microsc. 1996;10(4):1105-9; discussion 1109-10.
We have used atomic force microscopy (AFM) to study radiation-induced DNA double strand breaks. Double-stranded plasmid DNA was irradiated with 18-MeV electrons in aqueous buffer, using a medical linear accelerator. Doses of 50, 100, 150, and 200 Gy were delivered to DNA samples, and atomic force microscopy was used to measure the length of each DNA fragment. From these measurements, we obtained the average length of the irradiated DNA for each sample and found a linear-quadratic relationship between the average length and radiation dose.
我们利用原子力显微镜（AFM）来研究辐射诱导的DNA双链断裂。使用医用直线加速器，在水性缓冲液中用18 MeV电子照射双链质粒DNA。将50、100、150和200 Gy的剂量施加到DNA样品上，并用原子力显微镜测量每个DNA片段的长度。通过这些测量，我们获得了每个样品中辐照DNA的平均长度，并发现平均长度与辐射剂量之间存在线性二次关系。
