海人酸诱导的视网膜无长突样细胞损伤由AMPA受体介导。

Kainate-induced retina amacrine-like cell damage is mediated by AMPA receptors.

作者信息

Ferreira I L, Duarte C B, Carvalho A P

机构信息

Center for Neuroscience of Coimbra, Department of Zoology, University of Coimbra, Portugal.

出版信息

Neuroreport. 1998 Oct 26;9(15):3471-5. doi: 10.1097/00001756-199810260-00025.

DOI:10.1097/00001756-199810260-00025

PMID:9855301

Abstract

We investigated the effect of domoate, kainate and AMPA on 45Ca2+ uptake and on metabolic activity of cultured chick amacrine-like cells, as measured by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Domoate and kainate stimulated 45Ca2+ uptake and decreased MTT reduction, in a LY 303070-sensitive manner. AMPA caused a small increase on 45Ca2+ uptake, but it was without effect on MTT reduction. AMPA reduced both the 45Ca2+ entry and neurotoxicity induced by kainate, and cyclothiazide enhanced both the 45Ca2+ entry and neurotoxicity induced by AMPA. The results indicate that the AMPA receptors are the non-NMDA glutamate receptors involved in excitotoxicity.

摘要

我们研究了软骨藻酸、海人酸和α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）对培养的鸡类无长突细胞摄取45Ca2+以及代谢活性的影响，代谢活性通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）的还原进行测定。软骨藻酸和海人酸以对LY 303070敏感的方式刺激45Ca2+摄取并降低MTT还原。AMPA使45Ca2+摄取略有增加，但对MTT还原没有影响。AMPA减少了海人酸诱导的45Ca2+内流和神经毒性，而环噻嗪增强了AMPA诱导的45Ca2+内流和神经毒性。结果表明，AMPA受体是参与兴奋性毒性的非NMDA谷氨酸受体。