Danjo Y, Watanabe H, Tisdale A S, George M, Tsumura T, Abelson M B, Gipson I K
Schepens Eye Research Institute and the Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts 02114, USA.
Invest Ophthalmol Vis Sci. 1998 Dec;39(13):2602-9.
The objective of this study was to determine whether alteration in mucins could be detected in patients with dry eye symptoms by using the monoclonal antibody H185, which recognizes carbohydrate epitopes on mucin molecules.
Immunohistochemistry and immunoelectron microscopy was used to examine binding of H185 antibody to conjunctival cells obtained by nitrocellulose filter paper stripping (impression cytology). Two study populations were examined. Study I included 22 patients with dry eye symptoms and 13 normal volunteers. Study II included 16 aqueous-deficient dry eye patients and 14 age-matched control subjects.
Results of the studies demonstrated significant differences in binding patterns of H185 to conjunctival cells in normal eyes compared with those of patients with dry eye symptoms. In normal eyes, the antibody bound to apical cells in a mosaic pattern, with cells exhibiting either light, medium, or intense binding. A predominant pattern in patients with dry eye symptoms was loss of the mosaic pattern with replacement by a "starry sky" pattern in which there was a lack of apical cell binding (hence, dark sky) but increased binding to goblet cells (hence, stars in the sky). The starry sky pattern correlated with rose bengal staining.
From these studies it is concluded that there is an alteration either in mucin distribution or mucin glycosylation on the surfaces of apical conjunctival cells in dry eye and that glycosylation of goblet cell mucins changes with the disease.
本研究的目的是确定使用识别粘蛋白分子上碳水化合物表位的单克隆抗体H185，能否在有干眼症状的患者中检测到粘蛋白的改变。
采用免疫组织化学和免疫电子显微镜技术，检测H185抗体与通过硝酸纤维素滤纸剥离法（印迹细胞学）获得的结膜细胞的结合情况。研究了两个群体。研究I包括22例有干眼症状的患者和13名正常志愿者。研究II包括16例泪液分泌不足型干眼患者和14名年龄匹配的对照受试者。
研究结果表明，与有干眼症状的患者相比，正常眼中H185与结膜细胞的结合模式存在显著差异。在正常眼中，抗体以镶嵌模式与表层细胞结合，细胞表现出轻度、中度或强烈结合。干眼症状患者的主要模式是镶嵌模式消失，取而代之的是“星空”模式，即缺乏表层细胞结合（因此为暗天），但与杯状细胞的结合增加（因此为天空中的星星）。星空模式与孟加拉玫瑰红染色相关。
从这些研究得出结论，干眼患者结膜表层细胞表面的粘蛋白分布或粘蛋白糖基化存在改变，且杯状细胞粘蛋白的糖基化随疾病而变化。
