Sawicka-Grzelak A, Rokosz A
Zakład Bakteriologii Klinicznej AM w Warszawie.
Med Dosw Mikrobiol. 1998;50(1-2):69-76.
The aim of the study was to determine the activity of four beta-lactam antibiotics against nosocomial strains of Gram-positive bacteria. Two antibiotics combined with beta-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in surgical ward of the National Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 STAPH, API STREP, API CORYNE and API 20 A strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB STAPH, ATB STREP and ATB ANA strips. The susceptibility of strains to timentin, tazocin, imipenem and meropenem was tested with disc diffusion method. 111 strains of Gram-positive bacteria were cultured. Staphylococci (49) and enterococci (44) dominated among isolated strains. 33 Staphylococcus spp. strains were identified as methicillin-resistant. The obtained results indicate a significant role of Gram-positive cocci (staphylococci and enterococci) in the aetiology of nosocomial infections. Antibiotics combined with beta-lactamase inhibitors and carbapenems demonstrate broad antibacterial spectrum against clinical strains of Gram-positive bacteria except E. faecium strains.
该研究的目的是确定四种β-内酰胺类抗生素对革兰氏阳性菌医院菌株的活性。应用了两种与β-内酰胺酶抑制剂联合的抗生素：替门汀（替卡西林/克拉维酸）和他唑仙（哌拉西林/三唑巴坦），以及两种碳青霉烯类抗生素：亚胺培南和美罗培南。临床菌株从华沙第一国立临床医院外科病房住院患者中分离得到。使用ID 32 STAPH、API STREP、API CORYNE和API 20 A条带在自动ATB系统中对菌株进行鉴定。使用ATB STAPH、ATB STREP和ATB ANA条带在自动ATB系统中测定分离株对抗菌剂的敏感性。采用纸片扩散法检测菌株对替门汀、他唑仙、亚胺培南和美罗培南的敏感性。培养了111株革兰氏阳性菌。分离菌株中葡萄球菌（49株）和肠球菌（44株）占主导。33株葡萄球菌属菌株被鉴定为耐甲氧西林。所得结果表明革兰氏阳性球菌（葡萄球菌和肠球菌）在医院感染病因学中起重要作用。与β-内酰胺酶抑制剂联合的抗生素和碳青霉烯类抗生素对革兰氏阳性菌临床菌株具有广泛的抗菌谱，但对粪肠球菌菌株除外。
