CD40对淋巴瘤B细胞上含死亡结构域的受体及其配体的调控

CD40 regulation of death domains containing receptors and their ligands on lymphoma B cells.

作者信息

Ribeiro P, Renard N, Warzocha K, Charlot C, Jeandenant L, Callet-Bauchu E, Coiffier B, Salles G

机构信息

Service d'Hématologie, Centre Hospitalier Lyon-Sud, Hospices Civils de Lyon et UPRES-JE 1879 Hémopathies Lymphoïdes Malignes, France.

出版信息

Br J Haematol. 1998 Dec;103(3):684-9. doi: 10.1046/j.1365-2141.1998.01063.x.

DOI:10.1046/j.1365-2141.1998.01063.x

PMID:9858217

Abstract

Within the tumour necrosis factor (TNF) family the induction of apoptosis is restricted to some ligand-receptors pairs, including TNF-TNF receptor type I (TNFRI/p55), FasL-Fas, TNF-related apoptosis-inducing ligand (TRAIL) and its death-receptors (DR)-4 and -5. The pair CD40L-CD40 belongs to the same family but rescues B cells from apoptosis. To investigate how these opposing actions are cross-linked, purified follicular lymphoma (FL) cells were activated upon a human CD40L-transfected murine fibroblastic layer, then RNA messengers for the above molecules were analysed using RT-PCR. The observed down-modulation of TRAIL and up-regulation of TNF and Fas transcripts might account for CD40-CD40L-mediated FL cell survival.

摘要

在肿瘤坏死因子（TNF）家族中，细胞凋亡的诱导仅限于某些配体-受体对，包括TNF-Ⅰ型TNF受体（TNFRI/p55）、FasL-Fas、TNF相关凋亡诱导配体（TRAIL）及其死亡受体（DR）-4和-5。CD40L-CD40这一对属于同一家族，但可使B细胞免于凋亡。为了研究这些相反的作用是如何相互关联的，将纯化的滤泡性淋巴瘤（FL）细胞接种在转染了人CD40L的鼠成纤维细胞层上进行激活，然后使用逆转录聚合酶链反应（RT-PCR）分析上述分子的信使核糖核酸（RNA）。观察到的TRAIL下调以及TNF和Fas转录本上调可能是CD40-CD40L介导的FL细胞存活的原因。