Sourjik V, Sterr W, Platzer J, Bos I, Haslbeck M, Schmitt R
Lehrstuhl für Genetik, Universität Regensburg, D-93040, Regensburg, Germany.
Gene. 1998 Nov 26;223(1-2):283-90. doi: 10.1016/s0378-1119(98)00160-7.
Three previously identified gene clusters that contain chemotaxis (che), flagellar (fla) and motility (mot) genes of Sinorhizobium meliloti (formerly Rhizobium meliloti) were mapped to a contiguous 45-kb region of the S. meliloti RU11/001 genome by pulsed-field gel electrophoresis (PFGE) in combination with Southern hybridization. The entire region was cloned and sequenced. The map combines 32 che, fla (flg, flh, fli) and mot genes and nine new open reading frames that probably encode taxis-related functions as well. It is concluded that between 80 and 90% of the genes responsible for chemotaxis and motility are located in a single region of the S. meliloti chromosome near the his-39 marker.
通过脉冲场凝胶电泳（PFGE）结合Southern杂交，将先前鉴定出的三个基因簇定位到苜蓿中华根瘤菌（以前称为苜蓿根瘤菌）的趋化性（che）、鞭毛（fla）和运动性（mot）基因，该三个基因簇位于苜蓿中华根瘤菌RU11/001基因组的一个连续45 kb区域。对整个区域进行了克隆和测序。该图谱整合了32个che、fla（flg、flh、fli）和mot基因以及九个可能也编码与趋化性相关功能的新开放阅读框。得出的结论是，负责趋化性和运动性的基因中，80%至90%位于苜蓿中华根瘤菌染色体上靠近his-39标记的单个区域。
