Guan M, Romano G, Henderson E E
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Anticancer Res. 1998 Sep-Oct;18(5A):3171-80.
AIDS-related Epstein-Barr virus (EBV)-associated T cell lymphomas are emerging as a new, distinct histopathological entity. The pathway whereby EBV infects T cells as well as the initial EBV transcriptional program in T cells has not been established. In order to shed light on the early events of the EBV infection of T cells, we have used in situ reverse transcription based polymerase chain reaction (RT-PCR) to study the initial EBV transcriptional program in homogeneous CD4+ and CD8+ lymphocytes. Following EBV infection, Epstein-Barr nuclear antigen (EBNA) expression could be detected in T rosetting CD4+ and CD8+ T lymphocytes. Only a few cells showed viral capsid antigen (VCA). EBV immediate-early gene transcripts (BZLF1, BRLF1, and BMLF1) encoded in the BamHI Z, R, and M fragments could be detected by in situ RT-PCR in the EBV producer cell line B95.8. Both BZLF1 and BRLF1 immediate-early transcripts, but not BMLF1 transcript, could be detected in individual CD4+ and CD8+ T cells infected with EBV. Demonstration of EBV mRNA transcripts encoding immediate-early transcriptional transactivators in EBV-infected T cells provides the first evidence for a possible mechanism whereby EBV could contribute to T cell proliferation and EBV-associated T cell malignancies.
与艾滋病相关的爱泼斯坦-巴尔病毒（EBV）相关T细胞淋巴瘤正成为一种新的、独特的组织病理学实体。EBV感染T细胞的途径以及T细胞中最初的EBV转录程序尚未明确。为了阐明EBV感染T细胞的早期事件，我们使用基于原位逆转录的聚合酶链反应（RT-PCR）来研究纯合CD4+和CD8+淋巴细胞中最初的EBV转录程序。EBV感染后，在T花环形成的CD4+和CD8+ T淋巴细胞中可检测到爱泼斯坦-巴尔核抗原（EBNA）表达。只有少数细胞显示病毒衣壳抗原（VCA）。通过原位RT-PCR可在EBV产生细胞系B95.8中检测到BamHI Z、R和M片段中编码的EBV即刻早期基因转录本（BZLF1、BRLF1和BMLF1）。在感染EBV的单个CD4+和CD8+ T细胞中可检测到BZLF1和BRLF1即刻早期转录本，但未检测到BMLF1转录本。在EBV感染的T细胞中证明编码即刻早期转录反式激活因子的EBV mRNA转录本，为EBV可能促进T细胞增殖和EBV相关T细胞恶性肿瘤的机制提供了首个证据。
