Mazurek S, Grimm H, Wilker S, Leib S, Eigenbrodt E
Institute of Biochemistry and Endocrinology, Veterinary Faculty, University of Giessen, Germany.
Anticancer Res. 1998 Sep-Oct;18(5A):3275-82.
Extracellular AMP inhibits cell proliferation of MCF-7 and MDA-MB-453 whereas cell proliferation of the highly malignant Novikoff cell line is not affected. In medium with low glucose supply MDA-MB-453 cells grow well, Novikoff cells are slightly inhibited and MCF-7 cells are totally unable to grow. Isoelectric focusing revealed that a glyclytic enzyme complex exists in all three cell lines. In addition to the glycolytic enzymes, c-Raf-kinase, adenylate kinase, and nucleoside diphosphate kinase are also found within the complex. The differences in glucose in dependence of the three cell lines can be explained by the different constitutions of shuttle enzymes. MDA-MB-453 and Novikoff cells contain cytsolic glycerol 3-phosphate dehydrogenase which is associated with glyceraldehyde 3-phosphate dehydrogenase within the glycolytic enzyme complex and which is responsible for the transport of cytoslic hydrogen in the mitochondria. MCF-7 and Novikoff cells contain the pI 7.8 form of malate dehydrogenase which couples glycolysis with glutaminolysis.
细胞外AMP抑制MCF-7和MDA-MB-453的细胞增殖，而对高恶性的诺维科夫细胞系的细胞增殖没有影响。在低糖供应的培养基中，MDA-MB-453细胞生长良好，诺维科夫细胞略有抑制，而MCF-7细胞完全无法生长。等电聚焦显示，所有三种细胞系中都存在一种糖酵解酶复合物。除了糖酵解酶外，复合物中还发现了c-Raf激酶、腺苷酸激酶和核苷二磷酸激酶。三种细胞系对葡萄糖的不同依赖性可通过穿梭酶的不同组成来解释。MDA-MB-453和诺维科夫细胞含有胞质甘油3-磷酸脱氢酶，它与糖酵解酶复合物中的甘油醛3-磷酸脱氢酶相关，负责将胞质氢转运到线粒体中。MCF-7和诺维科夫细胞含有pI 7.8形式的苹果酸脱氢酶，它将糖酵解与谷氨酰胺分解偶联起来。
