Kata M, Baran L, Siwanowicz J, Dziedzicka-Wasylewska M
Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
Pol J Pharmacol. 1998 May-Jun;50(3):259-64.
The present study examined ex vivo effect of kainic acid and pentetrazole administration on the activity of nitric oxide synthase (NOS) in the mouse brain. NOS activity was assayed by measuring the formation of [3H] citrulline from [3H]arginine in the homogenates of mouse hippocampus, neocortex and cerebellum. The highest basal activity of the enzyme was found in this latter brain region. Administration of kainic acid (30 mg/kg) increased the NOS activity in all brain regions examined. On the other hand, pentetrazole (60 mg/kg) did not evoke any significant changes in the NOS activity at 5 min after the administration. Only in cerebellum, at 10 min after administration of pentetrazole, the increase in the activity of the enzyme was observed. The obtained results indicate that the two particular convulsants used in this study differ not only in respect of behavioral signs of seizures which they evoke, but also in respect of the effect on mouse brain NOS activity.
本研究检测了给予海藻酸和戊四氮对小鼠脑组织中一氧化氮合酶（NOS）活性的离体效应。通过测量小鼠海马、新皮层和小脑匀浆中[3H]精氨酸生成[3H]瓜氨酸的量来测定NOS活性。该酶的基础活性在后者（小脑）脑区中最高。给予海藻酸（30mg/kg）可增加所有检测脑区的NOS活性。另一方面，戊四氮（60mg/kg）给药后5分钟，未引起NOS活性的任何显著变化。仅在戊四氮给药后10分钟，在小脑中观察到该酶活性增加。所得结果表明，本研究中使用的两种特定惊厥剂不仅在所诱发的癫痫行为体征方面不同，而且在对小鼠脑NOS活性的影响方面也不同。
