Li C X, Li L, Lou J, Yang W X, Lei T W, Li Y H, Liu J, Cheng M L, Huang L H
Department of Pharmacology, Guiyang Medical College, Guizhou, China.
Am J Chin Med. 1998;26(3-4):325-32. doi: 10.1142/S0192415X98000361.
Han-Dan-Gan-Le, a Chinese medicine preparation composed of Salvia miltorrhiza, Radix paeoniae, Astragalus membranaceus, Stephania tetrandra, and dried leaves of Ginkgo biloba, has been used successfully to treat human liver fibrosis and cirrhosis for years. This study was designed to examine the mechanisms of the protection. Male Wistar rats were given CCl4 (1.2 ml/kg, 2 times/week), 20% fat diet, and 30% alcohol in drinking water (every other day) for 6 weeks. Han-Dan-Gan-Le (0.5 and 1.0 g/kg, p.o., daily for 6 weeks) was administered to rats simultaneously to examine the protective effects against CCl4-induced liver fibrosis. The experimentally-induced liver fibrosis and other morphological alterations were significantly ameliorated by Han-Dan-Gan-Le. Han-Dan-Gan-Le treatments decreased CCl4-induced hepatic collagen accumulation by more than 50%, and significantly increased urinary excretion of hydroxyproline. The CCl4-induced lipid peroxidation in liver and serum was ameliorated as a result of Han-Dan-Gan-Le treatment, possibly by restoring the activity of superoxide dismutase activity in liver and erythrocytes, In conclusion, Han-Dan-Gan-Le is effective in protecting against liver fibrosis. The mechanisms of the protection appear to be due to its antioxidant properties and the modulation of hepatic collagen metabolism.
肝丹肝乐是一种由丹参、白芍、黄芪、防己和银杏叶干燥叶组成的中药制剂，多年来已成功用于治疗人类肝纤维化和肝硬化。本研究旨在探讨其保护机制。雄性Wistar大鼠给予四氯化碳（1.2 ml/kg，每周2次）、20%高脂饮食和饮用水中30%乙醇（隔天一次），持续6周。同时给予大鼠肝丹肝乐（0.5和1.0 g/kg，口服，每日一次，共6周），以研究其对四氯化碳诱导的肝纤维化的保护作用。肝丹肝乐显著改善了实验诱导的肝纤维化和其他形态学改变。肝丹肝乐治疗使四氯化碳诱导的肝脏胶原积累减少了50%以上，并显著增加了尿羟脯氨酸的排泄。肝丹肝乐治疗可改善四氯化碳诱导的肝脏和血清脂质过氧化，可能是通过恢复肝脏和红细胞中超氧化物歧化酶的活性。总之，肝丹肝乐对肝纤维化具有有效的保护作用。其保护机制似乎是由于其抗氧化特性和对肝脏胶原代谢的调节作用。
