Mikhaĭlova A A, Zakharova L A
Biull Eksp Biol Med. 1976 Jun;81(6):700-2.
The radioisotopic method with the use of immunosorbents was applied to the study of the synthesis of antibodies and nonspecific immunoglobulins in the mono- and mixed cultures of cells of the immune lymph nodes and intact bone marrow in removal of the adherent or T-cells. Treatment of the lymph node cell population with anti-O-serum decreased the antibody synthesis to 30%, whereas removal of the adherent cells - to 70%. In combined cultivation of the cells of immune lymph nodes, devoid of adherent cells, with the intact bone marrow cells the effect of stimulation of immunoglobulin synthesis was not eliminated, whereas treatment of cells of the immune lymph nodes with anti-O-serum doubled this effect. Possible mechanisms of the effect of stimulation of the immunoglobulin synthesis in mixed culture is discussed, and a conclusion was drawn on the necessity of cooperation between the individual types of cells during the productive phase of the immune response.
采用免疫吸附剂的放射性同位素方法被应用于研究在去除黏附细胞或T细胞的情况下，免疫淋巴结和完整骨髓细胞的单培养及混合培养中抗体和非特异性免疫球蛋白的合成。用抗O血清处理淋巴结细胞群体可使抗体合成降至30%，而去除黏附细胞则可使其降至70%。在缺乏黏附细胞的免疫淋巴结细胞与完整骨髓细胞的联合培养中，免疫球蛋白合成的刺激作用并未消除，而用抗O血清处理免疫淋巴结细胞则使这种作用加倍。讨论了混合培养中免疫球蛋白合成刺激作用的可能机制，并得出结论：在免疫反应的产生阶段，不同类型细胞之间有合作的必要性。
