Ko Y L, Ko Y S, Wang S M, Hsu L A, Chang C J, Chu P H, Cheng N J, Chen W J, Chiang C W, Lee Y S
The First Cardiovascular Division, Chang-Gung Memorial Hospital, Taipei, Taiwan, ROC.
Atherosclerosis. 1998 Dec;141(2):259-64. doi: 10.1016/s0021-9150(98)00179-8.
Paraoxonase (PON1) is a high density lipoprotein-associated enzyme capable of hydrolyzing lipid peroxides, and thus, might protect lipoproteins from oxidation. A common polymorphism due to an amino acid substitution (Gln-Arg) at codon 191 is considered to be a major determinant of variation in serum PON1 activity. Recent studies have suggested that the PON1-191 polymorphism is an independent risk factor for coronary atherosclerosis in patients with or without diabetes mellitus. The association of PON1-191 polymorphism genotypes and coronary artery disease (CAD) among Chinese subjects in Taiwan was examined. The genotype of 218 angiographically documented CAD patients and the same number of age- and sex-matched control subjects was determined. Genotypes AA, AB and BB were present in 25 (11%), 102 (47%) and 91 (42%) of control subjects, respectively, and in 30 (14%), 96 (44%) and 92 (42%) of CAD patients, respectively (chi2 = 0.57, P = 0.75 between groups). The frequency of the A allele was 0.36 for the control group and 0.35 for CAD patients (P = 0.94). No significant differences in the PON1-191 genotype frequencies could be found between groups when multivariate logistic regression analysis was performed, or different subgroups of age, sex or risk factors were analyzed. Among control subjects, there was also no significant difference between genotypes of the PON1-191 polymorphism and various clinical and lipid variables. In conclusion, our data suggest that there is no association between the Gln-Arg 191 polymorphism of the human PON1 gene and CAD among Chinese subjects in Taiwan.
对氧磷酶（PON1）是一种与高密度脂蛋白相关的酶，能够水解脂质过氧化物，因此可能保护脂蛋白免受氧化。密码子191处由于氨基酸替代（谷氨酰胺-精氨酸）导致的常见多态性被认为是血清PON1活性变异的主要决定因素。最近的研究表明，PON1 - 191多态性是患有或未患有糖尿病的患者发生冠状动脉粥样硬化的独立危险因素。我们研究了台湾中国人群中PON1 - 191多态性基因型与冠状动脉疾病（CAD）之间的关联。确定了218例经血管造影证实的CAD患者以及相同数量的年龄和性别匹配的对照受试者的基因型。基因型AA、AB和BB在对照受试者中分别占25例（11%）、102例（47%）和91例（42%），在CAD患者中分别占30例（14%）、96例（44%）和92例（42%）（两组间χ2 = 0.57，P = 0.75）。对照组A等位基因频率为0.36，CAD患者为0.35（P = 0.94）。进行多因素逻辑回归分析时，或分析年龄、性别或危险因素的不同亚组时，两组间PON1 - 191基因型频率均无显著差异。在对照受试者中，PON1 - 191多态性的基因型与各种临床和脂质变量之间也无显著差异。总之，我们的数据表明，台湾中国人群中人类PON1基因的谷氨酰胺-精氨酸191多态性与CAD之间无关联。
