Graber P, Gretener D, Herren S, Aubry J P, Elson G, Poudrier J, Lecoanet-Henchoz S, Alouani S, Losberger C, Bonnefoy J Y, Kosco-Vilbois M H, Gauchat J F
Geneva Biomedical Research Institute, Plan-les-Ouates, Switzerland.
Eur J Immunol. 1998 Dec;28(12):4286-98. doi: 10.1002/(SICI)1521-4141(199812)28:12<4286::AID-IMMU4286>3.0.CO;2-H.
To study the expression of IL-13 receptor alpha1 (IL-13Ralpha1), specific monoclonal antibodies (mAb) were generated. Surface expression of the IL-13Ralpha1 on B cells, monocytes and T cells was assessed by flow cytometry using these specific mAb. Among tonsillar B cells, the expression was the highest on the IgD+ CD38- B cell subpopulation which is believed to represent naive B cells. Expression was also detectable on a large fraction of the IgD-CD38- B cells but not on CD38+ B cells. Activation under conditions which promote B cell Ig class switching up-regulated the expression of the receptor. However, the same stimuli had an opposite effect for IL-13Ralpha1 expression levels on monocytes. While IL-13Ralpha1 mRNA was clearly detectable in T cell preparations, no surface expression was detected. However, permeabilization of the T cells showed a clear intracellular expression of the receptor. A soluble form of the receptor was immunoprecipitated from the supernatant of activated peripheral T cells, suggesting that T cell IL-13Ralpha1 might have functions unrelated to the capacity to form a type II IL-4/IL-13R with IL-4Ralpha.
为研究白细胞介素-13受体α1（IL-13Rα1）的表达，制备了特异性单克隆抗体（mAb）。使用这些特异性mAb通过流式细胞术评估IL-13Rα1在B细胞、单核细胞和T细胞上的表面表达。在扁桃体B细胞中，IgD⁺CD38⁻B细胞亚群的表达最高，该亚群被认为代表幼稚B细胞。在大部分IgD⁻CD38⁻B细胞中也可检测到表达，但在CD38⁺B细胞中未检测到。在促进B细胞免疫球蛋白类别转换的条件下激活会上调受体的表达。然而，相同的刺激对单核细胞上IL-13Rα1的表达水平有相反的作用。虽然在T细胞制剂中可清楚检测到IL-13Rα1 mRNA，但未检测到表面表达。然而，T细胞的通透化显示受体有明显的细胞内表达。从活化的外周T细胞的上清液中免疫沉淀出受体的可溶性形式，表明T细胞IL-13Rα1可能具有与和IL-4Rα形成II型IL-4/IL-13R的能力无关的功能。
