Latchman Y, Reiser H
Department of Immunology, Imperial College School of Medicine, Hammersmith Hospital, London, GB.
Eur J Immunol. 1998 Dec;28(12):4325-31. doi: 10.1002/(SICI)1521-4141(199812)28:12<4325::AID-IMMU4325>3.0.CO;2-T.
The physiological functions of murine CD2 and its ligand CD48 are uncertain. We have examined the role of the CD2-CD48 interaction in murine T cell activation using a series of Chinese hamster ovary (CHO) cell transfectants. CHO cells expressing I-Ad together with CD48 induced more potent activation of OVA-specific, I-Ad-restricted DO11.10-transgenic T cells than CHO cells expressing I-Ad alone. CD48 augmented proliferation and IL-2 production in response to antigen. The enhancing effect of CD48 was of the same magnitude as that seen for CD80 (B7-1). Conjugate assays revealed the ability of CD48 to increase adhesion between T cells and CHO transfectants. The enhancing effects of CD48 on T cell-antigen-presenting cell adhesion and T cell activation were inhibited by anti-CD2 monoclonal antibody. This report provides the first evidence that the CD2 ligand CD48 contributes to the interactions of murine CD4+ T cells with antigen-presenting cells.
小鼠CD2及其配体CD48的生理功能尚不清楚。我们使用一系列中国仓鼠卵巢（CHO）细胞转染体研究了CD2-CD48相互作用在小鼠T细胞活化中的作用。与单独表达I-Ad的CHO细胞相比，同时表达I-Ad和CD48的CHO细胞能更有效地激活OVA特异性、I-Ad限制性的DO11.10转基因T细胞。CD48可增强抗原刺激后的增殖和IL-2产生。CD48的增强作用与CD80（B7-1）的作用强度相同。结合试验显示CD48能够增加T细胞与CHO转染体之间的黏附。抗CD2单克隆抗体可抑制CD48对T细胞-抗原呈递细胞黏附及T细胞活化的增强作用。本报告首次证明CD2配体CD48有助于小鼠CD4+T细胞与抗原呈递细胞之间的相互作用。
