Kimura F, Ishida S, Seto K, Kawakami M
Endokrinologie. 1976 Jun;67(2):172-83.
Fluctuations of ovarian biosynthetic activity and effects of exogenous LH and FSH on it during the estrous cycle were investigated by measuring in vitro transfer rates of 14C from 14C-1-acetate into progesterone (P), 20 alpha-hydroxy-pregn-4-en-3-one (20 alpha-OH-P) and estrogen (estradiol and estrone, E) in the ovarian homogenates from rats autopsied at 2 hour intervals. The transfer rate of 14C from 14C-1-acetate into P was lowest in the afternoon of estrus and increased from the morning of diestrus 1, making its peaks during the afternoon of diestrus 2 and in the midnight of proestrus. The transfer of 14C into 20 alpha-OH-P was high on the days of diestrus 2 and proestrus with its peak in the afternoon of the latter day. The maximum transfer of 14C into E in the afternoon of proestrus and a high rate in the morning of estrus with relatively low one in the midnight were observed. Exogenously injected LH (150 mug) or FSH (150 mug) was either stimulatory or inhiibitory to the transfer rates of 14C from 14C-1-acetate into ovarian steroids. During day time of diestrus 2 and midnight between proestrus and estrus, the transfer of 14C into P and 20 alpha-OH-P increased by LH, and during day time of proestrus and from the afternoon of estrus to the morning of diestrus 1 decreased. The transfer of 14C into E increased by LH from the afternoon of diestrus 2 to the morning of proestrus, and decreased during the afternoon of proestrus and from the afternoon of estrus to the morning of diestrus 2. Administration of FSH was also stimulatory or inhibitory. The 14C transfer into P and 20 alpha-OH-P increased by FSH from the afternoon of estrus to the morning of proestrus, but in the afternoon of proestrus they decreased. Transfer of 14C into E increased by FSH significantly on the days of diestrus 2 and proestrus, and slightly on the day of estrus, while it decreased in the afternoon of diestrus 1.
通过测定每隔2小时处死的大鼠卵巢匀浆中14C从14C-1-醋酸盐向孕酮（P）、20α-羟基孕-4-烯-3-酮（20α-OH-P）和雌激素（雌二醇和雌酮，E）的体外转移率，研究了发情周期中卵巢生物合成活性的波动以及外源性促黄体生成素（LH）和促卵泡生成素（FSH）对其的影响。14C从14C-1-醋酸盐向P的转移率在发情期下午最低，从间情期1上午开始升高，在间情期2下午和发情前期午夜达到峰值。14C向20α-OH-P的转移在间情期2和发情前期较高，在发情前期下午达到峰值。观察到14C在发情前期下午向E的转移量最大，在发情期上午转移率较高，在午夜相对较低。外源性注射LH（150微克）或FSH（150微克）对14C从14C-1-醋酸盐向卵巢类固醇的转移率有刺激或抑制作用。在间情期2白天以及发情前期和发情期之间的午夜，LH使14C向P和20α-OH-P的转移增加，而在发情前期白天以及从发情期下午到间情期1上午则减少。从间情期2下午到发情前期上午，LH使14C向E的转移增加，而在发情前期下午以及从发情期下午到间情期2上午则减少。FSH的施用也有刺激或抑制作用。从发情期下午到发情前期上午，FSH使14C向P和20α-OH-P的转移增加，但在发情前期下午则减少。在间情期2和发情前期，FSH使14C向E的转移显著增加，在发情期略有增加，而在间情期1下午则减少。
