苄青霉素在小鼠中诱导特异性非IgE抗体反应。

Benzylpenicillin induced specific non-IgE antibody response in mice.

作者信息

Zhang L, Wang Y M, Chen B Y, Shen G

机构信息

Department of Pharmacology, School of Pharmacy, Shanghai, China.

出版信息

Zhongguo Yao Li Xue Bao. 1996 Nov;17(6):531-4.

PMID:9863149

Abstract

AIM

To study whether or not the specific non-IgE antibody response in mice can be induced by benzylpenicillin in vivo.

METHODS

Antibody response and antigenic cross reactions were determined by enzyme-linked immunosorbent assay (ELISA). Antigen molecules recognized by antibodies were tested by hapten inhibition assay.

RESULTS

During d 1-d 50 after immunization, positive % of specific IgM, IgG, and IgA to benzylpenicillin were 100%, 50%-100%, and 17%-100%, respectively. IgM and IgG to benzylpenicillin also recognized ampicillin and piperacillin. The positive % of IgM and IgG to ampicillin were 23%-100% and 50%-100%, to piperacillin 43%-100% and 50%-100%, respectively. Aged benzylpenicillin showed an inhibitory effect on specific antibodies in a dose-dependent manner, and inhibitory % of specific IgM and IgG were 29%-87% and 29%-71%. However, freshly prepared benzylpenicillin had no effect.

CONCLUSION

Specific non-IgE antibody response was successfully induced by benzylpenicillin in mice, in which the isotypes were mainly IgM and then IgG and IgA. Antibodies recognized degraded products, not benzylpenicillin molecule itself. Antigenic cross reactions occurred between benzylpenicillin, ampicillin, and piperacillin. Isotypes of antibodies responsible for cross reactions were mainly IgG and then IgM.

摘要

目的

研究苄青霉素在体内是否能诱导小鼠产生特异性非IgE抗体反应。

方法

采用酶联免疫吸附测定法（ELISA）检测抗体反应和抗原交叉反应。通过半抗原抑制试验检测抗体识别的抗原分子。

结果

免疫后第1天至第50天，对苄青霉素特异性IgM、IgG和IgA的阳性率分别为100%、50% - 100%和17% - 100%。对苄青霉素的IgM和IgG也识别氨苄西林和哌拉西林。对氨苄西林IgM和IgG的阳性率分别为23% - 100%和50% - 100%，对哌拉西林的阳性率分别为43% - 100%和50% - 100%。老化的苄青霉素对特异性抗体有剂量依赖性抑制作用，特异性IgM和IgG的抑制率分别为29% - 87%和29% - 71%。然而，新制备的苄青霉素无此作用。