Qi J H, Zhang L, Wang J, Gu P K, Jin Z J, Huang M Z, Wang X M
Department of Pharmacology, Shanghai Second Medical University, China.
Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):448-50.
To observe the effect of captopril (Cap) on intracellular pH (pHi) in aortic smooth muscle cells (ASMC).
Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pHi was determined using digital image processing method.
The pHi of untreated SHR and WKY rats were 7.37 +/- 0.29 and 7.19 +/- 0.31, respectively. Oral Cap decreased pHi (7.11 +/- 0.26, P < 0.05) and exaggerated pHi response to angiotensin II (Ang-II, 0.1 mumol.L-1) in ASMC of SHR rats vs WKY rats (0.14 +/- 0.05 vs 0.21 +/- 0.05 pH units, P < 0.01). Cap in vitro had no effect on Ang-II induced intracellular alkalinization in ASMC of rabbits.
Oral Cap inhibits Na+(-)H+ exchange activity in ASMC of SHR rats.
