结肠CD44v6表达及其可溶性形式的血清浓度在溃疡性结肠炎与克罗恩病鉴别诊断中的诊断价值欠佳。

Poor diagnostic value of colonic CD44v6 expression and serum concentrations of its soluble form in the differentiation of ulcerative colitis from Crohn's disease.

作者信息

Reinisch W, Heider K H, Oberhuber G, Dejaco C, Müllner M, Adolf G R, Gasché C

机构信息

Department of Clinical Pharmacology, University of Vienna, Austria.

出版信息

Gut. 1998 Sep;43(3):375-82. doi: 10.1136/gut.43.3.375.

DOI:10.1136/gut.43.3.375

PMID:9863483

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1727246/

Abstract

BACKGROUND

Increased expression of CD44v6 on colonic crypt epithelial cells in ulcerative colitis has been suggested as a diagnostic tool to distinguish ulcerative colitis from colonic Crohn's disease.

AIMS

To investigate colonic CD44v6 expression and serum concentrations of soluble CD44v6 (sCD44v6) in patients with ulcerative colitis and Crohn's disease.

METHODS

Colonic biopsy samples were obtained from 16 patients with ulcerative colitis, 13 with ileocolonic Crohn's disease, and 10 undergoing polypectomy. Serum samples were obtained from 15 patients with active ulcerative colitis, 20 with active Crohn's disease, and 20 healthy donors. Colonic CD44v6 expression was evaluated immunohistochemically by monoclonal antibody 2F10 and the higher affinity monoclonal antibody VFF18. Serum sCD44v6 concentrations were measured by ELISA.

RESULTS

2F10 stained colonic epithelium of inflamed ulcerative colitis and Crohn's disease samples in 80% and 40% of cases, respectively, and VFF18 in 95% and 87%, respectively. Both monoclonal antibodies displayed a sensitivity and specificity of 60% and 87% to differentiate ulcerative colitis from colonic Crohn's disease. Serum concentrations of sCD44v6 were lower in patients with ulcerative colitis (median 153 ng/ml; interquartile range (IQR) 122-211) compared with Crohn's disease (219; IQR 180-243) and healthy donors (221; IQR 197-241 (p = 0.002)). Its sensitivity and specificity to discriminate ulcerative colitis from Crohn's disease was 75% and 71%, respectively.

CONCLUSION

Colonic CD44v6 and serum sCD44v6 concentrations do not facilitate reliable differential diagnosis between ulcerative colitis and Crohn's disease.

摘要

背景

溃疡性结肠炎结肠隐窝上皮细胞上CD44v6表达增加，已被提议作为区分溃疡性结肠炎与结肠克罗恩病的诊断工具。

目的

研究溃疡性结肠炎和克罗恩病患者结肠CD44v6表达及可溶性CD44v6（sCD44v6）的血清浓度。

方法

从16例溃疡性结肠炎患者、13例回结肠型克罗恩病患者和10例接受息肉切除术者获取结肠活检样本。从15例活动期溃疡性结肠炎患者、20例活动期克罗恩病患者和20名健康供者获取血清样本。采用单克隆抗体2F10和高亲和力单克隆抗体VFF18通过免疫组织化学法评估结肠CD44v6表达。采用酶联免疫吸附测定法测量血清sCD44v6浓度。

结果

2F10分别在80%的炎症性溃疡性结肠炎样本和40%的克罗恩病样本中对结肠上皮进行染色，VFF18分别在95%和87%的样本中染色。两种单克隆抗体区分溃疡性结肠炎与结肠克罗恩病的敏感性和特异性均为60%和87%。与克罗恩病患者（中位数219 ng/ml；四分位间距（IQR）180 - 243）和健康供者（221；IQR 197 - 241（p = 0.002））相比，溃疡性结肠炎患者血清sCD44v6浓度较低（中位数153 ng/ml；IQR 122 - 211）。其区分溃疡性结肠炎与克罗恩病的敏感性和特异性分别为75%和71%。