纤细短膜虫线粒体细胞色素的中点电位
Midpoint potentials of the mitochondrial cytochromes of Crithidia fasciculata.
作者信息
Kusel J P, Storey B T
出版信息
J Bacteriol. 1976 Aug;127(2):812-6. doi: 10.1128/jb.127.2.812-816.1976.
Abstract
The midpoint potentials of the mitochondrial respiratory chain cytochromes of the protozoan Crithidia fasciculata at pH 7.2, Em7.2, show great similarity to those measured in higher organisms. Values of Em7.2 for cytochromes a and a3 are +165 and +340 mV. Both c cytochromes have Em7.2 = +230 mV. There are two b cytochromes with the same spectral characteristics with Em7.2 = -20 and -135 mV. These values are compatible with two sites of energy conservation for oxidative phosphorylation in these mitochondria. All cytochrome components show potentiometric titrations with n = 1. There is a fluorescent flavoprotein in these mitochondria with Em7.2 = -40 mV and n =2, whose function is not known.
摘要
原生动物纤细短膜虫线粒体呼吸链细胞色素在pH 7.2时的中点电位（Em7.2）与在高等生物中测得的电位非常相似。细胞色素a和a3的Em7.2值分别为+165和+340 mV。两种c细胞色素的Em7.2均为+230 mV。有两种b细胞色素具有相同的光谱特征，Em7.2分别为-20和-135 mV。这些值与这些线粒体中氧化磷酸化的两个能量保守位点相符。所有细胞色素成分的电位滴定n值均为1。这些线粒体中有一种荧光黄素蛋白，Em7.2为-40 mV，n值为2，其功能尚不清楚。
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