Haidan A, Hilbers U, Bornstein S R, Ehrhart-Bornstein M
Department of Internal Medicine III, University of Leipzig, Germany.
Peptides. 1998;19(9):1511-7. doi: 10.1016/s0196-9781(98)00115-6.
VIP receptors are frequently overexpressed by various endocrine tumors. In this study the expression of VIP receptors in the human adrenocortical carcinoma cell line NCI-H295 and their involvement in the regulation of steroidogenesis was investigated. NCI-H295 cells express VIP1 and VIP2 receptors as demonstrated by RT-PCR, whereas they do not express VIP itself. The receptors are functionally coupled to steroidogenesis since VIP (10(-9) M to 10(-6) M) exerted a dose-dependent stimulatory effect on the release of aldosterone, cortisol, and DHEA. VIP increased ACTH-stimulated releases of aldosterone and cortisol. The proliferation rate of NCI-H295 cells was not affected by VIP. These data show that NCI-H295 cells express both forms of the VIP receptor and that VIP is involved in an ACTH-independent regulation of steroidogenesis in the adrenal tumor cells.
血管活性肠肽（VIP）受体在多种内分泌肿瘤中常过度表达。在本研究中，对人肾上腺皮质癌细胞系NCI - H295中VIP受体的表达及其在类固醇生成调节中的作用进行了研究。逆转录聚合酶链反应（RT - PCR）证明NCI - H295细胞表达VIP1和VIP2受体，而它们不表达VIP本身。这些受体在功能上与类固醇生成相关，因为VIP（10⁻⁹M至10⁻⁶M）对醛固酮、皮质醇和脱氢表雄酮（DHEA）的释放具有剂量依赖性刺激作用。VIP增加了促肾上腺皮质激素（ACTH）刺激的醛固酮和皮质醇释放。NCI - H295细胞的增殖速率不受VIP影响。这些数据表明NCI - H295细胞表达两种形式的VIP受体，且VIP参与肾上腺肿瘤细胞中不依赖ACTH的类固醇生成调节。
