Erb R E, Malven P V, Monk E L, Mollett T A, Smith K L, Schanbacher F L, Willett L B
J Dairy Sci. 1976 Aug;59(8):1420-8. doi: 10.3168/jds.S0022-0302(76)84380-9.
Concentrations of progesterone, estrogen, and prolactin in plasma were compared among lactations induced in 29 cows by daily subcutaneous injections of .25 mg progesterone and .1 mg estradiol-17beta per kg body weight for 7 days. Superior, median, and inferior lactations were identified by ranking both weightage adjusted maximum milk yields in 7 consecutive days (average 144 +/- 9 kg) and days for milk yield to increase from 5 to 10 kg/day (15 +/- 3 days). Superior and inferior of the former averaged 189 and 101 kg, and their latter averaged 2 and 42 days. Plasma hormones were measured on day 0 before first treatment (day 1), and on days 7, 14, 17, 21, 24, 28, and 35. Superior lactations were associated with below-average progesterone and estrogen in plasma on day 0, rapid decreases in progesterone after day 7 and in estrogen after day 14, and increased prolactin after 7. In comparison, median lactations were associated with elevated progesterone and estrogen after day 17, but prolactin was similar to that of superior lactations. Inferior lactations were associated with decreased prolactin in plasma from days 21 to 35. We hypothesized that (a) first treatment should start 3 to 8 days after estrus, (b) daily doses of estradiol-17beta should be decreased with progesterone unchanged for the 7 days, and (c) estradiol-17beta alone should be continued for 7 days to improve hormonally induced lactations.
