Brown D R, Reiner S L
Committee on Immunology, The University of Chicago, Illinois 60637, USA.
Infect Immun. 1999 Jan;67(1):266-70. doi: 10.1128/IAI.67.1.266-270.1999.
B-cell-to-T-cell signaling can shape helper T (Th) cell responses. During infection with Leishmania major, Th response is critical in determining the outcome of disease. Resistance depends on the generation of a protective Th1 response, while susceptibility is mediated by the generation of a Th2 response. In this study, we determined whether B cells are required for the development of polarized Th1 and Th2 responses during infection with L. major. Mice lacking B cells due to disruption of the immunoglobulin M locus (microMT) were infected with L. major, and disease progression and Th cell development were assessed. On the genetically resistant C57BL background, both wild-type and microMT mice controlled the infection and mounted a Th1 response. On the genetically susceptible BALB/c background, both wild-type and microMT mice were susceptible to infection and generated Th2 responses. Thus, during L. major infection, neither direct antigen presentation or costimulation by B cells nor antibody-mediated effector functions are essential for the development of polarized Th responses.
B细胞向T细胞的信号传导可塑造辅助性T（Th）细胞反应。在感染硕大利什曼原虫期间，Th反应对于决定疾病的转归至关重要。抵抗力取决于保护性Th1反应的产生，而易感性则由Th2反应的产生介导。在本研究中，我们确定了在感染硕大利什曼原虫期间，极化的Th1和Th2反应的发展是否需要B细胞。因免疫球蛋白M基因座（microMT）破坏而缺乏B细胞的小鼠感染了硕大利什曼原虫，并评估了疾病进展和Th细胞发育。在遗传抗性C57BL背景下，野生型和microMT小鼠均控制了感染并产生了Th1反应。在遗传易感的BALB/c背景下，野生型和microMT小鼠均易受感染并产生Th2反应。因此，在感染硕大利什曼原虫期间，B细胞的直接抗原呈递或共刺激以及抗体介导的效应功能对于极化Th反应的发展均不是必需的。
