鉴定出两个编码肺炎衣原体97至99千道尔顿外膜蛋白的新基因。
Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae.
作者信息
Knudsen K, Madsen A S, Mygind P, Christiansen G, Birkelund S
机构信息
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
出版信息
Infect Immun. 1999 Jan;67(1):375-83. doi: 10.1128/IAI.67.1.375-383.1999.
Abstract
Two genes encoding 97- to 99-kDa Chlamydia pneumoniae VR1310 outer membrane proteins (Omp4 and Omp5) with mutual similarity were cloned and sequenced. The proteins were shown to be constituents of the C. pneumoniae outer membrane complex, and the deduced amino acid sequences were similar to those of putative outer membrane proteins encoded by the Chlamydia psittaci and Chlamydia trachomatis gene families. By use of a monospecific polyclonal antibody against purified recombinant Omp4, it was shown that without heating, the protein migrated at 65 to 75 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoelectron microscopy showed that epitopes of Omp4 were exposed on the surface of C. pneumoniae elementary bodies, reticulate bodies, and outer membrane complex. Proteins encoded by the C. pneumoniae gene family seem to be dominant antigens in experimentally infected mice.
摘要
克隆并测序了两个编码相互相似的97至99 kDa肺炎衣原体VR1310外膜蛋白（Omp4和Omp5）的基因。这些蛋白被证明是肺炎衣原体外膜复合物的组成成分，推导的氨基酸序列与鹦鹉热衣原体和沙眼衣原体基因家族编码的假定外膜蛋白的序列相似。使用针对纯化重组Omp4的单特异性多克隆抗体，结果显示在不加热的情况下，该蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中迁移至65至75 kDa。免疫电子显微镜显示Omp4的表位暴露于肺炎衣原体原体、网状体和外膜复合物的表面。肺炎衣原体基因家族编码的蛋白似乎是实验感染小鼠中的主要抗原。
相似文献
1
Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae.
Infect Immun. 1999 Jan;67(1):375-83. doi: 10.1128/IAI.67.1.375-383.1999.
2
Potential relevance of Chlamydia pneumoniae surface proteins to an effective vaccine.
J Infect Dis. 2000 Jun;181 Suppl 3:S528-37. doi: 10.1086/315633.
3
Immunoreactivity of the 60 kDa cysteine-rich proteins of Chlamydia trachomatis, Chlamydia psittaci and Chlamydia pneumoniae expressed in Escherichia coli.
Microbiology (Reading). 1994 Aug;140 ( Pt 8):2003-11. doi: 10.1099/13500872-140-8-2003.
4
Molecular biology of Chlamydia pneumoniae surface proteins and their role in immunopathogenicity.
Am Heart J. 1999 Nov;138(5 Pt 2):S491-5. doi: 10.1016/s0002-8703(99)70283-8.
5
Characterization of Chlamydia pneumoniae species-specific proteins immunodominant in humans.
J Clin Microbiol. 1994 Mar;32(3):583-8. doi: 10.1128/jcm.32.3.583-588.1994.
6
Structural and antigenic analysis of Chlamydia pneumoniae.
Infect Immun. 1990 Jan;58(1):93-7. doi: 10.1128/iai.58.1.93-97.1990.
7
Detection of Chlamydia pneumoniae-specific antibodies binding to the VD2 and VD3 regions of the major outer membrane protein.
J Clin Microbiol. 2003 May;41(5):1957-62. doi: 10.1128/JCM.41.5.1957-1962.2003.
8
A secondary structure motif predictive of protein localization to the chlamydial inclusion membrane.
Cell Microbiol. 2000 Feb;2(1):35-47. doi: 10.1046/j.1462-5822.2000.00029.x.
9
Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci and Chlamydia pneumoniae.
FEBS Lett. 1999 Feb 19;445(1):192-6. doi: 10.1016/s0014-5793(99)00121-0.
10
Remarkable sequence relatedness in the DNA encoding the major outer membrane protein of Chlamydia psittaci (koala type I) and Chlamydia pneumoniae.
Gene. 1994 Jan 28;138(1-2):139-42. doi: 10.1016/0378-1119(94)90796-x.
引用本文的文献
1
Opsonophagocytosis of Chlamydia pneumoniae by Human Monocytes and Neutrophils.
Infect Immun. 2020 Jun 22;88(7). doi: 10.1128/IAI.00087-20.
2
Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia psittaci as Candidate Factors in Pathogenesis and Immunity to Infection.
PLoS One. 2016 Sep 15;11(9):e0162392. doi: 10.1371/journal.pone.0162392. eCollection 2016.
3
All subtypes of the Pmp adhesin family are implicated in chlamydial virulence and show species-specific function.
Microbiologyopen. 2014 Aug;3(4):544-56. doi: 10.1002/mbo3.186. Epub 2014 Jul 1.
4
Members of the Pmp protein family of Chlamydia pneumoniae mediate adhesion to human cells via short repetitive peptide motifs.
Mol Microbiol. 2010 Nov;78(4):1004-17. doi: 10.1111/j.1365-2958.2010.07386.x. Epub 2010 Oct 6.
5
Comprehensive in silico prediction and analysis of chlamydial outer membrane proteins reflects evolution and life style of the Chlamydiae.
BMC Genomics. 2009 Dec 29;10:634. doi: 10.1186/1471-2164-10-634.
6
Chlamydia pneumoniae GroEL1 protein is cell surface associated and required for infection of HEp-2 cells.
J Bacteriol. 2008 May;190(10):3757-67. doi: 10.1128/JB.01638-07. Epub 2008 Feb 29.
7
Characterization of in vitro chlamydial cultures in low-oxygen atmospheres.
J Bacteriol. 2007 Sep;189(18):6723-6. doi: 10.1128/JB.00279-07. Epub 2007 Jul 13.
8
Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair.
BMC Microbiol. 2003 Sep 2;3:19. doi: 10.1186/1471-2180-3-19.
9
The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029.
BMC Microbiol. 2002 Nov 26;2:36. doi: 10.1186/1471-2180-2-36.
10
Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae.
BMC Microbiol. 2002 Jul 9;2:17. doi: 10.1186/1471-2180-2-17.
本文引用的文献
1
Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface of Chlamydia psittaci.
FEMS Microbiol Lett. 1998 Jul 1;164(1):111-7. doi: 10.1111/j.1574-6968.1998.tb13075.x.
2
Molecular cloning and characterization of the genes coding for the highly immunogenic cluster of 90-kilodalton envelope proteins from the Chlamydia psittaci subtype that causes abortion in sheep.
Infect Immun. 1998 Apr;66(4):1317-24. doi: 10.1128/IAI.66.4.1317-1324.1998.
3
Antibody response to Chlamydia pneumoniae infection in children with respiratory illness.
J Infect Dis. 1998 Mar;177(3):720-4. doi: 10.1086/514223.
4
Chlamydia trachomatis utilizes the host cell microtubule network during early events of infection.
Mol Microbiol. 1997 Aug;25(3):441-9. doi: 10.1046/j.1365-2958.1997.4591832.x.
5
Molecular biology of the Chlamydia pneumoniae surface.
Scand J Infect Dis Suppl. 1997;104:5-10.
6
Localization by immunoelectron microscopy of antigens of Chlamydia psittaci suitable for diagnosis or vaccine development.
FEMS Microbiol Lett. 1997 May 1;150(1):113-9. doi: 10.1111/j.1574-6968.1997.tb10358.x.
7
Murine models of Chlamydia pneumoniae infection and atherosclerosis.
J Infect Dis. 1997 Apr;175(4):883-90. doi: 10.1086/513986.
8
Generation of Campylobacter fetus S-layer protein diversity utilizes a single promoter on an invertible DNA segment.
Mol Microbiol. 1996 Mar;19(6):1241-53. doi: 10.1111/j.1365-2958.1996.tb02469.x.
9
Characterization of two conformational epitopes of the Chlamydia trachomatis serovar L2 DnaK immunogen.
Infect Immun. 1996 Mar;64(3):810-7. doi: 10.1128/iai.64.3.810-817.1996.
10
Chlamydia pneumoniae (TWAR) in atherosclerosis of the carotid artery.
Circulation. 1995 Dec 15;92(12):3397-400. doi: 10.1161/01.cir.92.12.3397.
Zotero 插件