鉴定出两个编码肺炎衣原体97至99千道尔顿外膜蛋白的新基因。
Identification of two novel genes encoding 97- to 99-kilodalton outer membrane proteins of Chlamydia pneumoniae.
作者信息
Knudsen K, Madsen A S, Mygind P, Christiansen G, Birkelund S
机构信息
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
出版信息
Infect Immun. 1999 Jan;67(1):375-83. doi: 10.1128/IAI.67.1.375-383.1999.
Abstract
Two genes encoding 97- to 99-kDa Chlamydia pneumoniae VR1310 outer membrane proteins (Omp4 and Omp5) with mutual similarity were cloned and sequenced. The proteins were shown to be constituents of the C. pneumoniae outer membrane complex, and the deduced amino acid sequences were similar to those of putative outer membrane proteins encoded by the Chlamydia psittaci and Chlamydia trachomatis gene families. By use of a monospecific polyclonal antibody against purified recombinant Omp4, it was shown that without heating, the protein migrated at 65 to 75 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoelectron microscopy showed that epitopes of Omp4 were exposed on the surface of C. pneumoniae elementary bodies, reticulate bodies, and outer membrane complex. Proteins encoded by the C. pneumoniae gene family seem to be dominant antigens in experimentally infected mice.
摘要
克隆并测序了两个编码相互相似的97至99 kDa肺炎衣原体VR1310外膜蛋白（Omp4和Omp5）的基因。这些蛋白被证明是肺炎衣原体外膜复合物的组成成分，推导的氨基酸序列与鹦鹉热衣原体和沙眼衣原体基因家族编码的假定外膜蛋白的序列相似。使用针对纯化重组Omp4的单特异性多克隆抗体，结果显示在不加热的情况下，该蛋白在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中迁移至65至75 kDa。免疫电子显微镜显示Omp4的表位暴露于肺炎衣原体原体、网状体和外膜复合物的表面。肺炎衣原体基因家族编码的蛋白似乎是实验感染小鼠中的主要抗原。
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