HIV-1 基质蛋白（MA）、衣壳蛋白（CA）和核衣壳蛋白（NC）突变体的宿主细胞依赖性复制不依赖于病毒感染因子（Vif）和病毒蛋白U（Vpu）的功能。
Host cell-dependent replication of HIV-1 gag MA, CA, and NC mutants are independent of the functions of Vif and Vpu.
作者信息
Shimano R, Inubushi R, Adachi A
机构信息
Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
出版信息
Int J Mol Med. 1999 Jan;3(1):91-4. doi: 10.3892/ijmm.3.1.91.
We have previously shown that some gag gene mutants of human immunodeficiency virus type 1 (HIV-1) display a replication-defect in a cell-dependent manner. We and others have also demonstrated that the requirement of vif and vpu genes for HIV-1 replication is cell-dependent. To determine whether the cell-dependent growth of the HIV-1 gag mutants is related to the functions of Vif and Vpu, double mutants of gag-vif and gag-vpu were constructed, and monitored for their replication in various cell lines. The results obtained showed that the mutations in gag do not affect the cell-dependent functions of Vif and Vpu.
我们之前已经表明，人类免疫缺陷病毒1型（HIV-1）的一些gag基因突变体以细胞依赖的方式表现出复制缺陷。我们和其他人还证明，HIV-1复制对vif和vpu基因的需求是细胞依赖的。为了确定HIV-1 gag基因突变体的细胞依赖生长是否与Vif和Vpu的功能相关，构建了gag-vif和gag-vpu双突变体，并监测它们在各种细胞系中的复制情况。所得结果表明，gag基因的突变不会影响Vif和Vpu的细胞依赖性功能。
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