嗜热栖热菌B35的耐热DNA聚合酶：某些性质的分离与表征
Thermostable DNA-polymerase from Thermus thermophilus B35: isolation and characterization of some properties.
作者信息
Rechkunova N I, Akishev A G, Lebedeva N A, Khodyreva S N, Degtyarev S Kh, Lavrik O I
机构信息
Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
出版信息
Biochemistry (Mosc). 1998 Nov;63(11):1266-70.
Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermophilus B35 which was found in hot spring water. The enzyme with molecular mass 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hydroxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol seems to be more favorable due to higher temperature optimum of this enzyme and lack of restriction endonucleases in the initial strain.
耐热的嗜热栖热菌B35 DNA聚合酶是从温泉水中发现的嗜热栖热菌B35菌株中分离出来的。利用DEAE-琼脂糖、羟基磷灰石、己基琼脂糖和肝素-琼脂糖进行连续层析，分离出了分子量为87 kD的这种酶。嗜热栖热菌B35 DNA聚合酶的生化特性与从嗜热栖热菌HB8中分离出的嗜热栖热菌DNA聚合酶相似；然而，由于该酶的最适温度较高且初始菌株中缺乏限制性内切酶，嗜热栖热菌B35 DNA聚合酶的实际应用似乎更具优势。
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