Kozlov A P, Pliusnin A Z, Seĭts I F
Mol Biol (Mosk). 1976 Jan-Feb;10(1):78-83.
We have shown that 8S and U2 fractions of low molecular weight nuclear RNA lmwn RNA) have tow stable conformational states with different electrophoretic mobilities. We have shown also that fractions UIa and UIb are conformational isomers. Fractions 8S and U2 are present only in one conformational state during electrophoresis, while UI RNA is always represented by both conformations. The conformation of lmwn RNAs is dependent upon conditions of isolation of nuclei and of extraction of RNA. It may change during storage of water solution of RNA at -20 degrees. Conformational changes discovered are not the result of degradation. Only one of two stable conformations of any lmwn RNA may be physiologically active, as it is the case of 5S rRNA and tRNAS. One ought to keep this in mind while studying the functions of low molecular weight nuclear RNAs.
我们已经表明，低分子量核RNA（lmwn RNA）的8S和U2组分具有两种具有不同电泳迁移率的稳定构象状态。我们还表明，U1a和U1b组分是构象异构体。在电泳过程中，8S和U2组分仅以一种构象状态存在，而U1 RNA总是以两种构象形式存在。lmwn RNA的构象取决于细胞核分离和RNA提取的条件。在RNA水溶液于-20℃储存期间，其构象可能会发生变化。发现的构象变化不是降解的结果。任何lmwn RNA的两种稳定构象中只有一种可能具有生理活性，5S rRNA和tRNA的情况就是如此。在研究低分子量核RNA的功能时应该牢记这一点。
